Study shows how AF4-MALS-UV can separate and size large AAV aggregates

Postnova Analytics has published a new applications study that reports on how Asymmetric Flow Field Flow Fractionation (AF4) coupled with multi-angle light scattering (MALS) and Ultraviolet (UV) detectors can be used to quantify Adeno-Associated Virus (AAV) aggregation.

Study shows how AF4-MALS-UV can separate and size large AAV aggregates
Postnova AF2000

Adeno-associated viruses are gaining widespread popularity as vehicles for gene therapy due to their versatility and safety. However, one of the biggest concerns for manufacturing a uniform AAV suspension is the presence of viral aggregates, which can create problems with transduction efficiency, biodistribution, and immunogenicity. Due to their large size, often over 100 nm in diameter, AAV aggregates are challenging to separate and characterize by traditional column-based chromatography techniques.

Data is presented in this application study that demonstrates how AF4-MALS-UV can separate and size large AAV aggregates, and discern a difference in aggregate concentration due to the stressing protocol. By comparison, using traditional size exclusion chromatography techniques, the columns are often found to filter out some or all aggregates, resulting in incorrect determination of the aggregate content or even the false conclusion that no aggregates are present.

The Postnova AF2000 used in this study is a high-performance Asymmetric Flow Field-Flow Fractionation (AF4) platform for separation of nanoparticles and macromolecules in complex matrices. Modular in design, the AF2000 incorporates the combined experience, expertise and technological advances from Postnova Analytics' two decades of leadership in FFF. Incorporating a range of FFF modules in a single integrated system to provide universal separation, the AF2000 offers more flexibility, better performance and more robust results than any system before.

Citations

Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    Postnova Analytics. (2019, September 25). Study shows how AF4-MALS-UV can separate and size large AAV aggregates. News-Medical. Retrieved on October 30, 2020 from https://www.news-medical.net/news/20190925/Study-shows-how-AF4-MALS-UV-can-separate-and-size-large-AAV-aggregates.aspx.

  • MLA

    Postnova Analytics. "Study shows how AF4-MALS-UV can separate and size large AAV aggregates". News-Medical. 30 October 2020. <https://www.news-medical.net/news/20190925/Study-shows-how-AF4-MALS-UV-can-separate-and-size-large-AAV-aggregates.aspx>.

  • Chicago

    Postnova Analytics. "Study shows how AF4-MALS-UV can separate and size large AAV aggregates". News-Medical. https://www.news-medical.net/news/20190925/Study-shows-how-AF4-MALS-UV-can-separate-and-size-large-AAV-aggregates.aspx. (accessed October 30, 2020).

  • Harvard

    Postnova Analytics. 2019. Study shows how AF4-MALS-UV can separate and size large AAV aggregates. News-Medical, viewed 30 October 2020, https://www.news-medical.net/news/20190925/Study-shows-how-AF4-MALS-UV-can-separate-and-size-large-AAV-aggregates.aspx.

Comments

The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
You might also like... ×
Informative white paper demonstrates benefits of novel EAF4 technology