Exhaled breath may be a promising avenue for antibiotic monitoring

Exhaled breath may be very promising alternative to blood for the therapeutic monitoring of antibiotics, the ESCMID Global Congress (formerly ECCMID) in Barcelona, Spain (27-30 April) will hear.

Therapeutic drug monitoring (TDM) is used to monitor antibiotic concentrations in patients with severe infections and in patients in intensive care units. These patients may metabolise drugs differently and so may not respond to conventional doses of antibiotics (they require higher doses) or be more likely to develop toxic side-effects (they require lower doses). The TDM results allow the dosage to be tailored to their needs.

Currently, we use blood samples to do this. But the collection of blood is an invasive, time- and resource-consuming technique and leads to discomfort in patients.

It takes several hours or even days to get results, depending on the antibiotic tested, and so the results may come too late to guide the treatment of some of the sickest patients.

Using exhaled breath has several advantages: it is non-invasive, it is easy to collect and it is not associated with discomfort to the patient. It also has the potential to decrease the turnaround time, especially when combined with real-time analyses, when it could provide results in as little as ten minutes.

It is already used to monitor other drugs, such as antiseizure medicines, and we wanted to explore its potential in monitoring antibiotics."

Dr. Sarah Dräger, lead researcher of the Division of Internal Medicine, University Hospital Basel, Basel, Switzerland

Dr. Dräger and Professor Pablo Sinues, head of the Translational Medicine Research Group at the University Children's Hospital of Basel, conducted a prospective, single-center proof-of-concept study involving 10 patients (median age 63 years, 54.5% female) who received intravenous antibiotic treatment in 2022 or 2023.

They were being treated for respiratory infections (n=3), intravascular infections (n=3), abdominal infections (n=2), urinary tract infection (n=1) or skin and soft tissue infection (n=1) with meropenem (n=3), piperacillin/tazobactam (n=3), cefazolin (n=2), flucloxacillin (n=1) or ciprofloxacin (n=1).

Mass spectrometry was used to analyze samples of exhaled breath for exogeneous metabolites (breakdown products of the antibiotic) and endogenous metabolites (breakdown products from the body that are affected by the antibiotic).

With four antibiotics – meropenem, cefazolin, flucloxacillin and ciprofloxacin – it was possible to detect differences in the levels of specific metabolites. With one antibiotic, piperacillin/tazobactam, no clear signal was detected.

The hope is that by measuring levels of these metabolites it will be possible to monitor antibiotic concentrations without taking blood samples.

Dr Dräger says: "We were able to detect antibiotic-specific metabolites in exhaled breath in patients treated with antibiotics in four out of five antibiotics investigated.

"We aim to confirm these very promising results in a larger cohort of patients, as well as look at how they relate to blood plasma concentrations of antibiotics and patient outcomes."


The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
Post a new comment

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.

You might also like...
Advanced techniques reveal plectasin's velcro-like action against bacteria