a division of Pall Life Sciences (NYSE: PLL)
and a leading supplier of label-free technology that accelerates the
development of biotherapeutic and pharmaceutical products, today
announced the introduction of its Dip and Read™ Ni-NTA
biosensor for use on its Octet®
instrumentation platforms. The new biosensor is pre-immobilized with
novel nickel-charged tri-nitrilotriacetic (Tri-NTA) groups enabling an
easy, rapid and direct method for quantitation and kinetic analysis of
polyhistidine-tagged (HIS-tagged) biomolecules.
"Nickel provides an excellent binding medium for the stable capture of
HIS-tagged proteins as part of quantitation and characterization
studies," said Christopher Silva, ForteBio division's vice president of
marketing. "However, standard formats for using this medium have been
cumbersome, time-consuming and expensive. Now, our new Ni-NTA biosensor
enables scientists to quickly and easily analyze HIS-fusion proteins
with unsurpassed ease of use and workflow efficiency."
The Ni-NTA biosensor is designed for use in buffer and diluted complex
media. Each biosensor can also be regenerated up to 10 times via a
standard low-pH protocol in as little as two minutes for various
applications such as acquisition of replicate data (same ligand/analyte
pair) and "bucket-based" screening applications.
ForteBio's Octet family of products is based on the company's
proprietary BioLayer Interferometry (BLI) technology. It incorporates
disposable optical Dip and Read biosensors that measure multiple
interactions in parallel, without the use of detection agents. For
single-sample analysis, ForteBio recently launched its BLItz platform.
The new platform revolutionizes the use of label-free protein analysis
by making it more accessible and cost-effective for individual bench
scientists in biotherapeutic discovery, process development and academic