Huntington's disease (HD) is a fatal hereditary disease for which there is no cure. A novel study from Cedars-Sinai Medical Center, Los Angeles, using pluripotent stem cells advances understanding of how the disease develops and may help pave the way for identifying pathways for future treatments. Results are published in the Journal of Huntington's Disease.
HD is caused by an expansion of a repeating "CAG" triplet series in the huntingtin (HTT) gene, which results in a protein with an abnormally long polyglutamine sequence. Individuals with between 36-40 CAG repeats have a less severe form of HD with later onset and slower progression. HD is traditionally considered as an adult onset neurodegenerative disorder, but a small number of patients carry more than 60 CAG repeats, which causes a severe form with juvenile onset. The present study was designed to explore the potential neurodevelopmental aspects of the disease.
Investigators used induced pluripotent stem cells (iPSC) using five cell lines generated from juvenile HD and five controls to model the development of the region of the brain most affected by HD. Lead investigator Virginia B. Mattis, PhD, with The Board of Governors Regenerative Medicine Institute and Department of Biomedical Sciences; Cedars-Sinai Medical Center, Los Angeles when the study was conducted, explained:
Before the discovery of embryonic stem cells, only post-mortem human brain tissues were available for us to study, limiting investigation of the developmental pathophysiology of the disease. However, with the use of iPSC, somatic cells reprogrammed to embryonic state, means it is possible to focus on the origins of HD in human brain tissues generated in vitro.
Using immunocytochemistry and stereological quantification, the researchers studied the differentiation of human iPSCs towards striatal fate and characterization. There was a distinct delay in development of juvenile onset HD iPSC characterized by the increased percentage of nestin-expressing neural progenitor cells at 42 days of differentiation. Nestin is a neural progenitor marker that is expressed throughout the development of the central nervous system. A partial reversal of this phenotype was demonstrated using small molecules or anti-sense oligonucleotides.
This study provides evidence to support the hypothesis of delayed development phenotype in the juvenile onset HD patient striatum. After differentiating towards striatal fate, juvenile HD cultures showed a significantly higher percentage of nestin positive cells. HTT knockdown throughout the course of differentiation can reverse the phenotype. Inhibition of canonical Notch pathway (DAPT) reversed the elevated nestin phenotype. This approach of disease modelling presents an ideal opportunity to study the early disease onset and its progression and can provide insight to understanding of the developmental aspects of juvenile HD. Better understanding the origins of disease could aid in targeting pathways of therapeutic intervention in the future.
Virginia B. Mattis of Cedars-Sinai Medical Center, Los Angeles
HD is a fatal genetic neurodegenerative disease characterized by atrophy of certain regions of the brain. It causes the progressive breakdown of nerve cells in the brain. HD patients experience behavioral changes and uncontrolled movements. Symptoms include personality changes, mood swings and depression, forgetfulness and impaired judgment, and unsteady gait and involuntary movements (chorea). Every child of an HD parent has a 50% chance of inheriting the gene. Patients usually survive 10-20 years after diagnosis.
Mathkar, P. P. et al. (2019) Characterization of Neurodevelopmental Abnormalities in iPSC-Derived Striatal Cultures from Patients with Huntington’s Disease. Journal of Huntington's Disease. doi.org/10.3233/JHD-180333