New research has implications for developing cancer therapies targeting mutant p53 protein

The tumor suppressor protein p53 is mutated in more than half of all human cancers. Several drugs that potentially can restore mutant p53 to its normal cancer-killing function are in clinical investigation.

However, much remains to be learned about various mutations that lead to a "loss of function" in the protein and others that cause a putative malignant "gain of function," acceleration of cancerous growth and spread (metastasis), for example.

Several studies have observed a positive correlation between p53 mutations and aneuploidy, an abnormal number of chromosomes that also can contribute to tumorigenesis (malignant growth). One unanswered question is what role, if any, aneuploidy plays in mutant p54 gain of function.

Now researchers at Vanderbilt University Medical Center have discovered that aneuploidy drives gain-of-function phenotypes in cells expressing mutant p53. Their report, published Aug. 31 in the journal Nature Communications, has implications for developing therapies targeting mutant p53.

The concept of mutant p53 gain of function was introduced over 30 years ago, and since then, many publications have reported context-specific and conflicting evidence for oncogenic phenotypes arising from overexpression of the mutant p53 protein."

Jennifer Pietenpol, PhD, Study Corresponding Author and Director, Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center

"Our study demonstrates that the acquisition of aneuploidy can generate a variety of the previously ascribed mutant p53 phenotypes and provides a unifying mechanism that accounts for the wide array and context-specific nature of phenotypes previously attributed to p53 mutant proteins," said  Jennifer Pietenpol, who is also the  Executive Vice President for Research at VUMC.

Pietenpol, the Benjamin F. Byrd Jr. Professor of Oncology and holder of the Brock Family Directorship in Career Development, has made significant contributions to understanding the role that p53 plays in both health and disease.

In the current study, the researchers used the CRISPR-Cas9 gene-editing technology to develop two genetically identical epithelial cell line models containing p53 mutations.

They found in vitro gain-of-function phenotypes only in cell lines displaying increased aneuploidy. Gain of function was not dependent on the expression of mutant p53 proteins. Outcome data also revealed that individuals with aneuploid-high tumors had an unfavorable prognosis, regardless of the p53 mutation.

Addressing mutant p53 gain of function thus must take into account the role of chromosomal alterations, the researchers concluded.

Comments

The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
Post a new comment
Post

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.

You might also like...
New insights into the role of gamma-delta T cells across 33 cancer types revealed