A guide to avoiding contamination in your qPCR assays

Quantitative polymerase chain reaction (qPCR) is a highly sensitive technique that allows researchers to amplify specific DNA sequences from a minimal starting amount.

However, this sensitivity poses a challenge in terms of potential contamination. Even the smallest quantities of DNA fragments from the laboratory environment or previous qPCR experiments can inadvertently undergo amplification during the reaction, leading to misleading outcomes like false positives. Identifying and resolving contamination issues can be particularly difficult for individuals new to qPCR experimentation.

Once DNA contamination takes place, it cannot be eliminated or reduced. Hence, prevention becomes paramount. 

Whether you are looking to identify the origin of potential contamination or seeking to enhance the efficiency of your assay, this free guide from LGC Biosearch Technologies will help you assess and mitigate contamination in your qPCR experiments. 

Get this whitepaper to help you:

  • Map unexpected results to possible sources.
  • Determine where your qPCR assay is vulnerable to contamination.
  • Follow recommendations to mitigate identified vulnerabilities.
  • Master the use of controls in routine testing.

 

 

Image Credit: LGC Biosearch Technologies

 

Download this whitepaper

 

About LGC Biosearch Technologies

Together, we help you advance.

Whether you’re using marker-assisted selection to develop more nutritious and sustainable crops or developing the next generation of molecular diagnostic tests, clinical genomics assays, or novel oligo therapeutics, accurate and high-quality data is everything. And so is speed.

To help keep your mission-critical projects on target and moving quickly, LGC provides a large integrated portfolio of products and services to support your entire genomics workflow and help at every stage of your product development pipeline. From R&D through to large-scale commercialization, our products and services scale up when you do.

Watch the video to see how our unified genomics portfolio helps you discover more, speeding up your work to improve the human condition.
 

Sponsored Content Policy: News-Medical.net publishes articles and related content that may be derived from sources where we have existing commercial relationships, provided such content adds value to the core editorial ethos of News-Medical.Net which is to educate and inform site visitors interested in medical research, science, medical devices and treatments.

 

Last updated: Jun 29, 2023 at 8:32 AM

Citations

Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    LGC Biosearch Technologies. (2023, June 29). A guide to avoiding contamination in your qPCR assays. News-Medical. Retrieved on October 04, 2024 from https://www.news-medical.net/whitepaper/20230628/Controlling-contamination-in-qPCR-to-ensure-data-reliability.aspx.

  • MLA

    LGC Biosearch Technologies. "A guide to avoiding contamination in your qPCR assays". News-Medical. 04 October 2024. <https://www.news-medical.net/whitepaper/20230628/Controlling-contamination-in-qPCR-to-ensure-data-reliability.aspx>.

  • Chicago

    LGC Biosearch Technologies. "A guide to avoiding contamination in your qPCR assays". News-Medical. https://www.news-medical.net/whitepaper/20230628/Controlling-contamination-in-qPCR-to-ensure-data-reliability.aspx. (accessed October 04, 2024).

  • Harvard

    LGC Biosearch Technologies. 2023. A guide to avoiding contamination in your qPCR assays. News-Medical, viewed 04 October 2024, https://www.news-medical.net/whitepaper/20230628/Controlling-contamination-in-qPCR-to-ensure-data-reliability.aspx.

Other White Papers by this Supplier

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.