Interleukin 6 (IL-6) is a pro-inflammatory cytokine involved in bone metabolism, hematopoiesis, immune responses and embryonic development. High IL-6 is noted in patients with microbial infections and other inflammatory conditions.
Targeting IL-6 has significant clinical implications for managing, diagnosing and preventing infectious diseases.
The pro-inflammatory function of IL-6 has been controlled by a trans-signaling process involving soluble IL-6R (sIL-6R) and gp130 receptor. The production of sIL-6R is triggered by TNF-alpha and IL-1beta. These two pro-inflammatory cytokines provoke the proteolytic cleavage of IL-6R by triggering ADAM10 and ADAM17.
Dissimilar to the majority of the other soluble receptors (for example, IL-1RA and IL-18BP), sIL-6R is NOT an inhibitor of IL-6. Rather, it triggers IL-6 signaling by developing a complex with IL-6 and gp130.
Human IL-6R (Cat#: 10398-H08H) enhanced the IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 is typically 20-80 ng/mL. Image Credit: Sino Biological Inc
Sino Biological has come up with a panel of high-quality recombinant IL-6 and receptor proteins to aid the study of this cytokine.
Sino Biological has developed bioactive recombinant IL-6 cytokines from several species, like human, rat, mouse, pig and rhesus.
Featured IL-6 protein
Human IL-6 protein: 10395-HNAE
High-purity Low-endotoxin Tag-free
Image Credit: Sino Biological Inc
Bioactive recombinant IL-6 from multiple species
Table 1. Source: Sino Biological Inc
| Species |
Cat# |
Activity Validated |
| Human |
10395-HNAE |
Bind to Human IL-6R Cell proliferation |
| Mouse |
50136-MNAE |
Cell proliferation |
| Rat |
80076-RNAE |
Cell proliferation |
| Rhesus |
90197-CNAE |
Cell proliferation |
| Sus scrofa (Pig) |
62006-WNAE |
Bind to Human IL-6R (10398-H08H) |
Recombinant membrane-bound IL-6 receptor: IL-6R
IL-6 illustrates restricted cross-species bioactivity. Human IL-6 has the ability to attach to both human and mouse IL-6R, but mouse IL-6 can only fix to mouse IL-6R.
Table 2. Source: Sino Biological Inc
| . |
. |
| Human IL-6 Receptor |
10398-H08H |
| Mouse IL-6 Receptor |
50280-M08H |
| Rat IL-6 Receptor |
81072-R08H |
| Canine IL-6 Receptor |
70117-D08H |
| Rhesus IL-6 Receptor |
90324-C08H |
Recombinant gp130
Gp130 is a general receptor and signal transducer shared by the majority of the IL-6 family cytokines.
Such as:
- Recombinant Rat gp130
- Recombinant Human gp130
- Recombinant Rhesus gp130
- Recombinant Mouse gp130
More IL-6 family cytokines
- IL-11
- IL-27
- NNT-1
- CT-1
- OSM
- CNTF
- IL-31
- LIF
Antibodies for IL-6 and receptors
Sino Biological has developed an extensive panel of antibodies that target IL-6 cytokines and receptors. This could be utilized in WB, ELISA, IP, and IHC-P.
Neutralization antibodies: IL-6 and IL-6R
IL-6 neutralizing antibody (Cat#: 10395-R508)
Image Credit: Sino Biological Inc
It is possible to neutralize IL-6 induced proliferation in the T1165 mouse plasmacytoma cells.
IL-6R neutralizing antibody (Cat#: 10398-MM08)
Image Credit: Sino Biological Inc
IL-6-dependent inhibition of cell proliferation has been neutralized by Human IL-6R neutralizing Monoclonal Antibody.
ELISA kits and ELISA detection pairs for IL-6 and receptors
- gp130 Detection Pairs
- IL-6 Detection Pairs
- IL-6R Detection Pairs
- IL-6 ELISA Kit
- IL-6R ELISA Kit
- gp130 ELISA Kit