IFN-γ: A therapeutic target for cancer therapy

Interferon gamma (IFN-γ) is a cytokine that induces a series of immune responses against viruses and tumors. The pro-apoptotic, anti-tumor and anti-proliferative actions of the cells are activated when IFN-γ binds to the IFN-γ receptor, which is made up of IFNGR1 and IFNGR2.

IFN-γ expression that is erroneous encourages the growth of tumors. IFN-γ has thus become recognized as a major contributor to the immunogenicity of malignancy.

The CD4+ T helper cells and CD8+ cytotoxic T cells are the effector T cells that release IFN-γ. Natural killer cells, natural killer T cells, and B cells are additional immune cells that secrete IFN-γ.

IFN-γ is stimulated by interleukins such IL-12, IL-15, IL-18, and IL-21. These interleukins’ stimulatory effects also result from intracellular signaling of transcription factors such as AP-1, JAK/STAT, and T-bet.

In PD-1/PD-L1 immune checkpoint inhibitor cancer treatment, IFN-γ is a prognostic marker. The production of IFN-γ is associated with an improved response to immune checkpoint blockade (ICB) therapy in triple-negative breast cancer, and is considered to be an emerging high-potential adjuvant therapy target.

Recombinant IFN-γ and Receptor Proteins

To facilitate the study of IFN- in cancer immunity, Sino Biological has produced a panel of recombinant IFN-γ protein and receptor reagents.

Featured IFN-γ and Receptor Proteins

Human IFN-γ Protein Cat#: 11725-HNAE

  • Tag-Free
  • HPLC-verified
  • High Activity
  • High-Purity

IFN-γ: A therapeutic target for cancer therapy

Purity: ≥ 95% as determined by SDS-PAGE & HPLC. Image Credit: Sino Biological Inc.

IFN-γ: A therapeutic target for cancer therapy

Anti-viral assays using WISH cells infected with vesicular stomatitis virus (VSV). Image Credit: Sino Biological Inc.

Human IFNGR1 Protein Cat#: 10338-H08H

IFN-γ: A therapeutic target for cancer therapy

Ability to inhibit rhIFN-γ mediated protection of WISH cells infected with vesicular stomatitis virus (VSV). Image Credit: Sino Biological Inc.

Mouse IFN-γ Protein Cat#: 50709-MNAH

IFN-γ: A therapeutic target for cancer therapy

Immobilized Mouse Interferon Gamma can bind Mouse IFNGR1 (Cat: 50705-M02H). Image Credit: Sino Biological Inc.

IFN-γ: A therapeutic target for cancer therapy

Antiviral assays using L929 cells infected with vesicular stomatitis virus (VSV). Image Credit: Sino Biological Inc.

Human IFNGR2 Protein Cat#: 50665-M08H

IFN-γ: A therapeutic target for cancer therapy

Purity: > 97 % as determined by SDS-PAGE. Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method. Image Credit: Sino Biological Inc.

Product list of Recombinant IFN-γ & Receptors

Table 1. Source: Sino Biological Inc.

Cat# Molecule Species Expressed Host Purity Activity Tag
11725-HNAE IFNG Human E. coli ≥95 % (by SEC-HPLC) Active Native
11725-HNAS Human CHO Stable Cells >92% Active Native
50709-M02H Mouse HEK293 Cells >92% Active C-hFc
50709-M08H Mouse HEK293 Cells >75% Active C-His
50709-MNAH Mouse HEK293 Cells >85% Active Native
60007-F08H Ferret HEK293 Cells >95%   C-His
80234-R02H Rat HEK293 Cells >92% Active C-hFc
90008-C08H Rhesus HEK293 Cells >90% Active C-His
90008-CNAE Rhesus E. coli >90% Active Native
10338-H02H IFNGR1 Human HEK293 Cells >95%   C-hFc
10338-H08H Human HEK293 Cells >97% Active C-His
50705-M02H Mouse HEK293 Cells >95% Active C-hFc
50705-M08H Mouse HEK293 Cells >98% Active C-His
80182-R02H Rat HEK293 Cells >95% Active C-hFc
90207-C02H Cynomolgus HEK293 Cells >95% Active C-hFc
90207-C08H Cynomolgus HEK293 Cells >95%   C-His
50665-M08H IFNGR2 Mouse HEK293 Cells >97%   C-His

 

Other related proteins

  • PD-1
  • CD4
  • IL-12
  • PD-L1
  • IL-18
  • CD8
  • IL-15
  • IL-21

Antibodies for IFN-γ and Receptors

For use in ELISA, WB, FCM, and IHC, Sino Biological has created a panel of antibodies that target the IFN-γ cytokine and its receptors.

Anti-IFN-γ Antibody (FITC), Rabbit Mab Cat#: 11725-R003-F

IFN-γ: A therapeutic target for cancer therapy

Flow cytometric analysis of Human IFN-γ expression on human peripheral blood lymphocytes. Image Credit: Sino Biological Inc.

Anti-IFN-γ Antibody, Rabbit PAb Cat#: 105908-T08

IFN-γ: A therapeutic target for cancer therapy

Immunochemical staining of human IFNG in human kidney cell. Image Credit: Sino Biological Inc.

Anti-IFNGR1 Antibody (APC), Mouse MAb Cat#: 10338-MM05-A

IFN-γ: A therapeutic target for cancer therapy

Flow cytometric analysis of Human IFNGR1 expression on human whole blood lymphocytes. Image Credit: Sino Biological Inc.

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.