Potentiated CD16 activated ADCC CAR-NK cells

In humans, there are two types of FcγR: the activating receptor, which contains the CD64 (FcγRI), CD32a (FcγRIIA), CD32c (FcγRIIC), CD16a (FcγRIIIA), and CD16b (FcγRIIIB); and the inhibiting receptor, CD32b (FcγRIIB). Table 1 lists the types and characteristics of FcγRs.

Table 1. The types and characteristics of FcγRs. Source: Sino Biological Inc.

Name FcγRI/CD64 FcγRIIA/CD32a FcγRIIB/CD32b FcγRIIC/CD32c FcγRIIIA/CD16a FcγRIIIB/CD16b
Structure Potentiated CD16 activated ADCC CAR-NK cells Potentiated CD16 activated ADCC CAR-NK cells Potentiated CD16 activated ADCC CAR-NK cells Potentiated CD16 activated ADCC CAR-NK cells Potentiated CD16 activated ADCC CAR-NK cells Potentiated CD16 activated ADCC CAR-NK cells
Function Activating Activating Inhibitory Activating Activating Activating
Affinity High Low Low Low Low Low
Effect of Antibody Binding ADCP, cytokine release ADCC, ADCP, vaccinal effect Inhibits ADCC, ADCP, B cell activation Enhances ADCC, ADCP, B cell activation ADCC, ADCP Decoy receptor that inhibits ADCP

 

To mediate antibody-dependent cellular cytotoxicity (ADCC), NK cells recognize cell-bound antibodies via CD16A (FcγRIIIA). A new area of cancer immunotherapy is enhancing therapeutic mAb-mediated CD16 activation of NK cells.

CAR-NK cells containing multi-specific molecules made up of relatively stable CD16 and other activating receptors (e.g., NKG2D, DNAM-1/CD226, NKp46/NCR1, NKp44/NCR2, and NKp30/NCR3), scFvs for tumor antigens (e.g., CD19, CD22, ROBO1, and PSMA), and cytokine moieties (e.g. CD16 genetic modifications to eliminate sensitivity to ADAM-17-mediated proteolytic cleavage or increase affinity for IgG Fc region have shown promising results.

CAR-NK cells expressing CD16A in recombination with the high-affinity Fc receptor, CD64 (FcγRI), demonstrate a more stable and enhanced ADCC response, indicating a promising advancement in CAR-NK cell immunotherapy.

Potentiated CD16 activated ADCC CAR-NK cells

Figure 1. Mechanism of CAR-NK cell-mediated ADCC. Image Credit: Sino Biological Inc.

Figure 1 depicts the concept of using CAR-NK cells in combination with antibody immunotherapy to counteract NK cell impairment, in which CAR-NK cells can kill target cancer cells independently via CAR and also serve as CD16-positive effector cells for mitigating ADCC against cancer cells.

Sino Biological has created a collection of high-quality proteins and antibodies for CD16 and related molecules to aid in CAR-NK cell therapy research.

Featured recombinant CD16 and CD64 proteins

Sino Biological provides cancer immunotherapy research with CD16a, CD16b, CD64, and related protein reagents.

Potentiated CD16 activated ADCC CAR-NK cells

Image Credit: Sino Biological Inc.

Human CD16a protein. Cat#: 10389-H08H1

Potentiated CD16 activated ADCC CAR-NK cells

High-Purity: >95% as determined by SEC-HPLC. Image Credit: Sino Biological Inc.

Potentiated CD16 activated ADCC CAR-NK cells

Immobilized Human CD16a (V176) can bind human IgG1 (Cat#: 10702-HNAC). Image Credit: Sino Biological Inc.

Human CD16b protein. Cat#: 11046-H08H1

Potentiated CD16 activated ADCC CAR-NK cells

High-Purity: >90 % as determined by SEC-HPLC. Image Credit: Sino Biological Inc.

Potentiated CD16 activated ADCC CAR-NK cells

BLI assay: Load IgG1 can bind human CD16b (NA1) recombinant protein. Image Credit: Sino Biological Inc.

Human CD64 protein. Cat#: 10256-H08H

Potentiated CD16 activated ADCC CAR-NK cells

High-Purity: >90 % as determined by SEC-HPLC. Image Credit: Sino Biological Inc.

Potentiated CD16 activated ADCC CAR-NK cells

Immobilized Anti-CD20 (Ro) antibody (Cat#: 68055-H005) can bind human CD64 protein. Image Credit: Sino Biological Inc.

More recombinant FcγR proteins (partial)

Table 2. Source: Sino Biological Inc.

Cat# Molecule Species Expression Host Purity Activity Tag
10389-H08C1 CD16a Human CHO Cells > 95% Active C-His
10389-H08H1 Human HEK293 Cells > 95% Active C-His
10389-H27H1-B Human HEK293 Cells > 95% Active C-AVI & His
80017-R08H Rat HEK293 Cells > 97% Active C-His
90013-C08H1-B Cynomolgus HEK293 Cells > 90%   C-His
11046-H08H1 CD16b Human HEK293 Cells > 95% Active C-His
11046-H27H1-B Human HEK293 Cells > 95% Active C-AVI & His
11046-HNAE-B Human E. coli > 90% Active Native
10374-H08H CD32a Human HEK293 Cells > 95% Active C-His
10374-H27H1-B Human HEK293 Cells > 95% Active C-AVI & His
10259-H08C CD32b Human CHO Cells > 95% Active C-His
10256-H08H CD64 Human HEK293 Cells > 95% Active C-His
10256-H08S Human CHO Stable Cells > 95% Active C-His
10256-H27H-B Human HEK293 Cells > 95% Active C-AVI & His
50086-M08H Mouse HEK293 Cells > 95% Active C-His
50086-M27H-B Mouse HEK293 Cells > 95% Active C-AVI & His
65010-T08H Rabbit HEK293 Cells > 90%   C-His
80016-R08H Rat HEK293 Cells > 95% Active C-His

 

Other related proteins

  • CD95
  • TROP-2
  • TRAIL
  • CD19
  • CTLA-4
  • NKG2C
  • IFN gamma
  • NKG2A
  • NKp80/KLRF1
  • KLRB1F
  • TIM3
  • PD1

Featured CD16 and CD64 antibodies

Sino Biological has created a collection of antibodies for CD16a, CD16b, CD64, and related molecules for use in FCM, ELISA, IHC-P, IP, and WB.

Anti-CD16a antibody (FITC). Mouse monoclonal. Cat#: 10389-MM41-F

Potentiated CD16 activated ADCC CAR-NK cells

Flow cytometric analysis of Human CD16 expression on human whole blood lymphocyte. Image Credit: Sino Biological Inc.

Anti-CD16b antibody. Mouse monoclonal. Cat#: 11046-MM07.

Potentiated CD16 activated ADCC CAR-NK cells

Immunochemical staining of human CD16b in human spleen. Image Credit: Sino Biological Inc.

Anti-CD64 antibody. Rabbit monoclonal. Cat#: 50086-R001.

Potentiated CD16 activated ADCC CAR-NK cells

Immunochemical staining of mouse CD64 in mouse liver. Image Credit: Sino Biological Inc.

More FcγR antibodies (partial)

Table 3. Source: Sino Biological Inc.

Cat# Antigen Species Clonality Ig Type Application
10389-MM22 CD16 Human Mouse MAb Mouse IgG1 FCM
10389-R221 Human Rabbit MAb Rabbit IgG FCM
10389-MM02 CD16a Human Mouse MAb Mouse IgG1 ELISA
10389-MM04 Human Mouse MAb Mouse IgG1 IHC-P
10389-MM41-F Human Mouse MAb Mouse IgG1 FCM
11046-MM01 CD16b Human Mouse MAb Mouse IgG2a ELISA
10374-MM01-F CD32a Human Mouse MAb Mouse IgG1 FCM
10259-MM02 CD32b Human Mouse MAb Mouse IgG1 ELISA
10259-RP03 Human Rabbit PAb Rabbit IgG ELISA
10256-MM06 CD64 Human Mouse MAb Mouse IgG1 ELISA
10256-R401 Human Rabbit MAb Rabbit IgG FCM
10256-R407 Human Rabbit MAb Rabbit IgG ELISA, FCM
10256-R407-F Human Rabbit MAb Rabbit IgG FCM
10256-RP01 Human Rabbit PAb Rabbit IgG ELISA
10256-RP02 Human Rabbit PAb Rabbit IgG WB, ELISA
50086-R001 Mouse Rabbit MAb Rabbit IgG ELISA, IHC-P
50086-R027 Mouse Rabbit MAb Rabbit IgG FCM, ICC/IF

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