At ASMS 2011, Bruker is introducing the breakthrough, proprietary CaptiveSpray electrospray ion source for nano-HPLC applications in proteomics. Using CaptiveSpray technology in many cases increases bottom-up protein identifications significantly, and CaptiveSpray is presently the best available technology for robust, reproducible protein ID or quantitative proteomics applications, with excellent, stable sensitivity over long time periods.
Unlike a traditional pulled nanospray tip, the Etch-TaperTM technology employed by CaptiveSpray ensures that the internal diameter of the spray tip remains constant, thereby reducing tip clogging, and providing excellent spray stability over the entire LC gradient and robust operation for long time periods, even with heavy proteomics samples loads. A key proprietary feature of the CaptiveSpray is its novel gas-flow focusing technology for dramatic sensitivity gains compared to normal electrospray. The CaptiveSpray source delivers nanospray sensitivity without the need for complex and time consuming spray tip adjustments, while its innovative plug-and-play design fits all current Bruker LC-MS instruments, including the latest maXis UHR-Qq-TOF systems, solariX FTMS systems and amaZon ETD ion trap mass spectrometers.
The novel CaptiveSpray source is uniquely designed to operate with LC flow rates from as low as 50 nL/min up to 5 uL/min and higher. This wide LC flow range allows analysts to significantly reduce column loading times, minimize column re-equilibration, and dramatically increase overall MS utilization time. The ability to run at higher LC flow rates, without compromising sensitivity, also increases sample loading capacity and expands the dynamic range of analysis. This combination of attributes provided by the unique CaptiveSpray source enhances the overall ability of the MS system to identify and quantify low-level peptides.
Dr. Kerry Nugent, Senior Vice President for Liquid Chromatography at Bruker's Chemical & Applied Markets Division, commented: "With the introduction of the CaptiveSpray ion source, Bruker has taken another step to enhance our leadership position in proteomics. The ability of the CaptiveSpray source to deliver a robust consistent spray, regardless of gradient composition or flow rate, makes it the ideal high-sensitivity MS source for applications that require a high level of accuracy and precision, such as bottom-up proteomics, peptide ID and protein quantification."
Study reveals breakthrough in non-invasive detection of endometrial cancer