Virtual Lens series of label-free live cell imaging systems announced by Phasefocus

Phase Focus Ltd (Phasefocus), the company that is revolutionising microscopy and imaging with the Phasefocus Virtual Lens®, announces the availability of the Virtual Lens series of label-free live cell imaging systems.

SpeClicks.aspx

Dr Rakesh Suman, KTP Associate in the Imaging & Cytometry unit at the University of York using the Phasefocus Virtual Lens system.

Phase Focus Ltd (Phasefocus) announces a family of innovative phase microscopy systems for live cell imaging using the Phasefocus Virtual Lens®. The Virtual Lens, VL, acquisition and processing engine uses a technique known as ptychography, to provide quantitative, high contrast, label-free images of cells for long term time-lapse studies.

The use of fluorescent probes is commonplace for labelling fixed specimens. However, when used in living cells, these can be toxic and ultimately perturb the natural cell function. The use of laser illumination can also be phototoxic to cells. The Phasefocus VL20 upright and VL21 inverted live cell imaging systems use very low power laser illumination thereby enabling long term time-lapse cell imaging, including multi-area time-lapse, to produce high contrast quantitative image information.

In addition to this, the quantitative nature of the phase information means that data such as cell thickness, cell volume, and cell motility can be measured on a familiar and easy-to-use system for the study of both individual cells and cell populations.

The Virtual Lens is based on technology developed at the University of Sheffield by Professor John Rodenburg. It provides an iterative solution of the diffraction pattern phase problem and is applicable to visible light imaging, X-ray imaging and atomic resolution electron imaging. Phasefocus has applied the technology to develop and install two systems at the University of York in the Department of Biology as one of many technology-leading techniques in the Imaging and Cytometry group managed by Dr Peter O'Toole.

Dr O'Toole describes ptychography as "one of the most important breakthroughs in imaging. It addresses many of the fundamental problems inherent in current microscopy techniques. Put simply, what you get is more than what you see! Artefacts of sample preparation which may arise from labelling or use of markers are negated. I have been most impressed by Phasefocus and their ability to quickly respond to feedback and then to develop products to meet the needs of the user."

In conjunction with the development of the Virtual Lens, Phasefocus is supporting a KTP Associate at York, - Dr Rakesh Suman - who works in Dr O'Toole's group tutoring members of the department in the use of the technique for the study of many different sample types including cancer and stem cells and applications relating to immunology and neurology.

For more information on ptychography and its application for label-free, live cell imaging, please visit the Phasefocus web site: http://www.phasefocus.com/.

Citations

Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    Phase Focus Limited. (2017, August 03). Virtual Lens series of label-free live cell imaging systems announced by Phasefocus. News-Medical. Retrieved on May 18, 2024 from https://www.news-medical.net/news/20140204/Virtual-Lens-series-of-label-free-live-cell-imaging-systems-announced-by-Phasefocus.aspx.

  • MLA

    Phase Focus Limited. "Virtual Lens series of label-free live cell imaging systems announced by Phasefocus". News-Medical. 18 May 2024. <https://www.news-medical.net/news/20140204/Virtual-Lens-series-of-label-free-live-cell-imaging-systems-announced-by-Phasefocus.aspx>.

  • Chicago

    Phase Focus Limited. "Virtual Lens series of label-free live cell imaging systems announced by Phasefocus". News-Medical. https://www.news-medical.net/news/20140204/Virtual-Lens-series-of-label-free-live-cell-imaging-systems-announced-by-Phasefocus.aspx. (accessed May 18, 2024).

  • Harvard

    Phase Focus Limited. 2017. Virtual Lens series of label-free live cell imaging systems announced by Phasefocus. News-Medical, viewed 18 May 2024, https://www.news-medical.net/news/20140204/Virtual-Lens-series-of-label-free-live-cell-imaging-systems-announced-by-Phasefocus.aspx.

Comments

The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
Post a new comment
Post

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.

You might also like...
Cornell University researchers achieve world's highest resolution images using ptychography