The Hong Kong Polytechnic University (PolyU) has achieved a breakthrough in the cancer genomics by developing a novel big data analysis platform for analyzing the interactions among genes. The analysis platform unveils the unregulated patterns of gene network in cancer and discovers potential diagnostic and therapeutic target genes, Nucleophosmin (NPM1) and its associated genes, in Chronic Myelogenous Leukemia (CML). These discoveries help the future development of NPM1-oriented treatment strategy in CML and enable targeted therapy to be more targeted. The analysis platform can also be readily applied to other diseases. The breakthrough has been made by a research team led by chair professor Benjamin Yung, which consists of biomedical scientists, Dr. Lawrence Chan and Dr. Cesar Wong from Department of Health Technology and Informatics (HTI) at PolyU and biostatisticians from Harvard University.
Traditional way to identify cancer gene markers is to examine genes in the whole human genome one-by-one with respect to their individual expressional differences between cancer patients and healthy people. Such deep-rooted method cannot provide any information about the interactions among genes and their systemic changes that compose the complicated cancer mechanism.
The research team hypothesized that genes participating in the same mechanism are co-expressed (i.e. the expression levels of gene pairs are correlated), and explored pair-to-pair correlations between genes so as to decipher the mechanism underlying the cancer. Combinations of gene pairs increase geometrically with the number of genes of interest. As the human genome has more than 20,000 protein-coding genes, the co-expression analysis could involve about 0.2 billion possible gene pairs. Moreover, the statistical test for co-expression analysis has to be refined to maintain the reliability of the detection results. Biostatistics experts of Harvard University helped the team perform a large-scale computer simulation to establish the statistical method for analyzing cancer-specific difference in co-expression. The team overcame challenges in processing massive data, and accomplished a performance of analyzing 0.2 billion gene pairs in two days, a mission impossible by the traditional method. The team has built an unprecedented scientific foundation of co-expression analysis, paving the way to the future of translational medicine.
This study has assured a novel structural co-expression network analysis platform, which unveils cancer pathogenesis and its potential NPM1-oriented treatment strategy. Co-expression analysis discovers novel unregulated patterns of gene network for understanding cancer biology, identifying new targets for treatment and all these innovations contribute to great science. This platform can be readily applied to other diseases for diagnostic, prognostic and therapeutic investigation. This finding will be published shortly in Scientific Reports from the publishers of Nature, showcasing the success of scientific research as a result of cross disciplinary collaboration.
The Hong Kong Polytechnic University