SARS-CoV-2 infections may induce neuroinflammation

In a recent study published in Cell Reports, researchers assessed the impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on neuroinflammation.

Study: SARS-CoV-2 infects neurons and induces neuroinflammation in a non-human primate model of COVID-19. Image Credit: Korawat photo shoot/Shutterstock
Study: SARS-CoV-2 infects neurons and induces neuroinflammation in a non-human primate model of COVID-19. Image Credit: Korawat photo shoot/Shutterstock

The causal virus of coronavirus disease 2019 (COVID-19), SARS-CoV-2, can cause many neurological problems in some patients. Yet, it is still unclear whether SARS-CoV-2 directly affects the brain or if central nervous system (CNS) sequelae result from systemic inflammatory reactions elicited by peripheral triggers.

About the study

In the present study, researchers employed a non-human monkey model for COVID-19 to examine whether SARS-CoV-2 penetrates the brain and the impact of aging on viral neurotropism.

This study used 14 colony-bred male and female rhesus macaques (Macaca mulatta) of Indian origin. The two main age groups for the animals were young adults aged between four and 12 years and older subjects aged 18 to 24. Three subgroups of young adults and older subjects were identified: infected non-type-2 diabetics, non-infected type-2 diabetics, and non-infected non-diabetics. 

Clinical observation includes keeping track of a patient's response, discharge, breathing rate and pattern, coughing and sneezing, appetite, and stool quality was assessed. Furthermore, rectal temperature, sp02, respiration, heart rate, and skin turgor were among the additional clinical evaluations performed. The investigation was further expanded to incorporate more brain areas and several viral markers. The team assessed neuroinflammation in the COVID-19 rhesus model developed in the study.

Subsequently, the piriform cortex was studied since it consistently showed the most significant levels of SARS-CoV-2 spike (Spk) and double-stranded ribonucleic acid (dsRNA). The team proposed that SARS-CoV2 neuroinflammation could cause cellular and synaptic damage.

Results

Cell-type analysis showed that in the young as well as old animals, the SARS-CoV-2 nucleocapsid (N) immunosignal colocalized with astrocytes (GFAP+), microglia (Iba1+), and neurons (NeuN+). Neurons had the highest level of intracellular N protein irrespective of age. At the same time, elderly animals revealed higher quantities than young animals across all three cell types, according to volume quantification of intracellular N protein by cell type. These findings imply that SARS-CoV-2 exhibits some neurotropism and that the infection burden rises with age.

Spk protein, as well as dsRNA, an intermediary component in the SARS-CoV-2 replication cycle that acts as a surrogate for productive infection, were also detected with SARS-CoV-2 N. Immunolabeling for these markers was found in multiple areas of the primary olfactory cortex, including the olfactory tubercle, the piriform cortex, and the olfactory pole of the entorhinal cortex, irrespective of age. In aged animals, SARS-CoV-2 viral markers were also observed in the orbitofrontal cortex, which is part of the secondary olfactory cortex. In contrast, only minimal labeling was observed outside the primary olfactory cortex in young animals.

The data showed that SARS-CoV-2 was neurotropic and could be identified in the macaque brain's olfactory regions within seven days post-infection (dpi). Although both glial and neuronal cells have viral proteins, productive infection is predominantly neuronal. Aged T2D mice showed more significant cellular changes in response to the virus as well as increased viral loads in all areas analyzed.

When young, infected animals were compared to their corresponding age-matched, non-infected controls, there was a rise in the number of astrocytes. Notably, GFAP populations significantly increased in both young and old infected mice, suggesting either astrocyte growth or translocation to the piriform cortex.

Additionally, concerning the young and infected group, microglial proliferation/translocation showed a considerable increase among aged and infected animals. These findings point to a significant inflammatory response to SARS-CoV-2 infection that involves the mobilization of both astrocytic and microglial cascades. Interestingly, only the older mice had inflammatory changes linked to diabetes, as evidenced by assessing the piriform cortex of diabetic young and elder control animals.

Human leukocyte antigen-D-related+ (HLA-DR) microglia displayed significant correlations with degraded myelin essential protein (dgMBP) instead of regular MBP expression, which could result in white-matter injury. Furthermore, HLA-DR+ microglia were in contact with neurons that expressed major histocompatibility complex (MHC) class I, which was a viral antigen that was significantly elevated in neurons present in diabetic older animals but worsened after SARS-CoV-2 infection. The team also noted a higher frequency associated with microglia’s abnormal morphology among infected animals. Notably, these microglia were found in the vicinity of neurons having morphological alterations, thus indicating a significant impact that suggested a neurodegenerative process.

Compared to young infected animals, there was a considerable drop in the density of PSD95+ puncta in older infected mice. The total microglia cellular volume was also higher in aged, infected animals as compared to young and infected subjects as well as aged, non-infected controls. Furthermore,  the total amount of internalized pan-Neurofilament (pan-NF) components increased in elderly, infected microglia.

Overall, the study findings noted that the prevalence of productive neuronal infection, along with the limited distribution of SARS-CoV-2 viral proteins into the olfactory circuit, indicated rapid transneuronal viral spread across corticocortical pathways, resulting in its dissemination in the CNS through the olfactory connectome.

Journal reference:
Bhavana Kunkalikar

Written by

Bhavana Kunkalikar

Bhavana Kunkalikar is a medical writer based in Goa, India. Her academic background is in Pharmaceutical sciences and she holds a Bachelor's degree in Pharmacy. Her educational background allowed her to foster an interest in anatomical and physiological sciences. Her college project work based on ‘The manifestations and causes of sickle cell anemia’ formed the stepping stone to a life-long fascination with human pathophysiology.

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