How to use a pipette for volatile liquids

It can be challenging to accurately pipette volatile liquids, such as ethanol, since they evaporate much faster than aqueous solutions. This can lead to dripping tips, which most people with no prior experience with pipetting volatile liquids experience.

When working with volatile liquids, it is good practice to pre-wet the tip before pipetting; using the liquid primed for pipetting, aspirate and dispense the entire volume of the tip three times to help release the air pressure and humidity that accumulates inside the tip.

Methods for achieving optimal aspirating and dispensing speeds can vary from liquid to liquid; however, it is recommended to work quickly after aspiration with all volatile liquids and pipette into the target vessel as soon as possible. Reverse pipetting, or discarding waste dispense once the pipetting of a volatile liquid is complete, is an effective technique that can be implemented if the results are significant.

How to use a pipette: Volatile liquids

Video Credit: INTEGRA Biosciences

About INTEGRA Biosciences

INTEGRA provides innovative solutions for Liquid Handling and Media Preparation applications which serve the needs of their customers in research, diagnostics and quality control laboratories.

Their instruments and plastic consumables are developed and manufactured in Zizers, Switzerland and Hudson, NH USA. In order to remain close to their customers, they maintain a direct sales and support organization in North America, the UK, France and Germany, as well as a network of over 100 highly trained distribution partners worldwide.

In recent years they have focused on developing a new and technologically advanced range of handheld electronic pipettes which are simple to use and meet the ergonomic needs of their customers.

Today they are proud to offer the widest range of electronic pipettes in the market spanning a range from single channel pipettes up to 384 channel bench-top instruments.

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Last updated: Nov 15, 2022 at 6:29 AM


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