LaVision BioTec reports on the research work of the Milan-based Iannacone Laboratory to study virus responses using intravital microscopy

LaVison BioTec, developers of advanced microscopy solutions for the life sciences, report on the research of Dr Matteo Iannacone of the San Raffaele Scientific Institute in Milan where intravital microscopy is being applied to the study of host-viruses and associated immune responses.

Matteo Iannacone, MD, PhD is the Group Leader in the Division of Immunology at the San Raffaele Scientific Institute, Milan, Italy. His research program seeks to dissect the complex dynamics of host-virus interactions with a particular focus on the development and function of adaptive immune responses. Since it is still beyond the reach of even the most sophisticated in vitro methodology to simulate the complex interplay of physical, cellular, biochemical and other factors that influence cell behavior in microvessels and interstitial tissues, the group uses intravital microscopy. This technique is complemented by more traditional molecular, cellular and histological approaches, thus characterizing host-virus interactions at the molecular-, single cell- and whole animal-level.

The Intravital Microscopy facility incorporating the LaVision BioTec's TriM Scope 2-photo microscope in the Iannacone Laboratory

The Intravital Microscopy facility incorporating the LaVision BioTec's TriM Scope 2-photo microscope in the Iannacone Laboratory

Discussing the selection of instrumentation, Dr Iannacone said “The choice of the TriM Scope from LaVision BioTec was driven by the desire of the group to couple a state-of-the-art multiphoton microscope with the possibility of customizing the entire system to our need to work in vivo. In these studies, both confocal immunofluorescence histology and correlative light-electron microscopy are used too. Overall, it was possibility of performing in vivo imaging that pushed us to our choice of the TriM Scope.”

Dr Iannacone is developing a new method where he will use the TriM Scope to map blood flow velocity. In vivo microscopy allows mapping of blood flow velocity at a high spatial and time resolution in the complex network of vessels constituting the hepatic microcirculation while simultaneously maintaining the morphologic information related to the dynamic parameters of vessels and immune cells. This on-going project with the Department of Physics at the University of Milano-Bicocca is reported online by Dr Iannacone's recent short communication published in Cellular & Molecular Immunology. This considers the potential of various novel imaging techniques to show how CD8+ T-cells move through the liver and how this may impact on the pathogenesis of Hepatitis B Virus, HBV.

For more details about LaVision BioTec's TriM Scope 2-photon Microscope and its applications, please contact LaVision BioTec on +49 (0)5219151390, visit the web site: www.lavisionbiotec.com.

Reference: Hepatic effector CD8+ T-cell dynamics: Cellular & Molecular Immunology advance online publication, 22 September 2014; doi:10.1038/cmi.2014.78

About LaVision BioTec GmbH

LaVision BioTec was founded in 2000 to develop and manufacture advanced microscopy solutions for the life sciences. There are currently two product lines:

TriM Scope II is a modular multi-photon/confocal microscopy platform that combines single- and multi-beam operation in one microscope. This allows for deep in-vivo imaging with Ti:Sapphire, OPO and visible lasers simultaneously with frame rates up to 60 Hz. PMTs, TCSPC and CCD detectors, multicolour imaging, spectral discrimination, FLIM/FRET capabilities and adaptive optics provide customization of the TriM Scope.

UltraMicroscope II utilizes six thin light sheets to excite samples with fluorescence light which is detected with a sCMOS-equipped microscope mounted perpendicular to the plane of illumination. Moving the sample through the light sheets generates 3D image stacks at cellular resolution.

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