Research reveals novel mechanism of ribosome dimerization using cryo-electron microscopy

Antibiotics are the most common medication used to treat microbial infections. Many antibiotics target intracellular bacterial ribosomes - cellular factories that synthesize proteins - which are essential for bacterial survival and proliferation. When bacteria have an excess of protein synthesis activity they stall the ribosomes in an inactive dimeric complex (i.e. two copies of ribosomes interact with each other). This so-called hibernating ribosome complex is more resistant to antibiotics.

In a collaborative effort, research groups from the Groningen Biomolecular Sciences and Biotechnology Institute of the University of Groningen led by Egbert Boekema, Bert Poolman and Albert Guskov revealed a novel mechanism of ribosome dimerization in the bacterium Lactococcus lactis using cryo-electron microscopy. The peculiarity of the mechanism they describe is that it involves a single protein, named HPFlong, which is capable to dimerize on its own and then pull two copies of ribosomes together. The dimeric state of the ribosome is no longer capable of synthesizing new proteins.

This hibernation mechanism is in a stark contrast with previous studies done in another microorganism, Escherichia coli. However based on a phylogenetic analysis of the amino acid sequence of HPFlong, the researchers conclude that the mechanism they propose is more widely spread, since protein HPFlong is present in nearly all known bacteria. This study provides the necessary structural basis to design new generations of antibiotics targeting hibernating ribosomes.

Comments

The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
Post a new comment
Post

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.

You might also like...
Optimizing research with automation: Solutions and best practices