Mutant bacterial receptor could help identify therapeutic compounds that inhibit quorum sensing

Researchers have developed a new mutant version of a receptor used by a bacterial pathogen for a chemical communication process called quorum sensing, according to a study published June 13 in the open-access journal PLOS Pathogens by Bonnie Bassler of Princeton University, and colleagues. As the authors note, the mutant receptor could be used to identify therapeutic compounds that inhibit quorum sensing, fulfilling an urgent medical need.

Mutant bacterial receptor could help identify therapeutic compounds that inhibit quorum sensing
RhlR* stimulates colony biofilm formation in P. aeruginosa in the absence of an autoinducer. Colony biofilm phenotypes of the designated P. aeruginosa strains following growth for 120 hours on tryptone Congo-red medium. Scale bar is 2 mm for all images. Images are representative of 3 biological replicates and 2 technical replicates for each biological replicate. Credit: McCready AR, et al. (2019)

The human pathogen Pseudomonas aeruginosa uses quorum sensing to orchestrate group behaviors, including biofilm formation and the production of infection-promoting molecules called virulence factors. Quorum sensing, which is essential for P. aeruginosa to be a pathogen, relies on the production, release, and detection of extracellular signal molecules called autoinducers. Autoinducers are bound by partner receptor proteins, and together, autoinducer-receptor complexes activate the transcription of quorum-sensing genes. In the new study, Bassler and colleagues identify, purify, and characterize a mutant version of the P. aeruginosa RhlR quorum-sensing receptor, which they call RhlR*.

Remarkably, RhlR* does not require its partner autoinducer to function. P. aeruginosa carrying RhlR* can properly form biofilms, produce virulence factors, and infect roundworms. Because RhlR* does not rely on an autoinducer to function, biochemical and genetic analyses that were previously not possible with RhlR could be performed with RhlR*. The findings provide new insight into the workings of other P. aeruginosa quorum-sensing components, most notably, the PqsE enzyme that functions together with RhlR to control virulence factor production. The authors propose that RhlR* is an especially valuable tool for learning about cell-cell communication and virulence in P. aeruginosa, a pathogen of high clinical relevance.

The authors add:

To our knowledge, this is the first report of a quorum-sensing receptor of this class that functions without its partner autoinducer molecule, opening up entirely new possibilities for study of virulence in Pseudomonas aeruginosa, a globally important pathogen.”

Source:

PLOS

Journal reference:

McCready, A.R. et al. (2019) An autoinducer-independent RhlR quorum-sensing receptor enables analysis of RhlR regulation. PLOS Pathogens. doi.org/10.1371/journal.ppat.1007820.

Citations

Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    PLOS ONE. (2019, June 14). Mutant bacterial receptor could help identify therapeutic compounds that inhibit quorum sensing. News-Medical. Retrieved on July 19, 2019 from https://www.news-medical.net/news/20190614/Mutant-bacterial-receptor-could-help-identify-therapeutic-compounds-that-inhibit-quorum-sensing.aspx.

  • MLA

    PLOS ONE. "Mutant bacterial receptor could help identify therapeutic compounds that inhibit quorum sensing". News-Medical. 19 July 2019. <https://www.news-medical.net/news/20190614/Mutant-bacterial-receptor-could-help-identify-therapeutic-compounds-that-inhibit-quorum-sensing.aspx>.

  • Chicago

    PLOS ONE. "Mutant bacterial receptor could help identify therapeutic compounds that inhibit quorum sensing". News-Medical. https://www.news-medical.net/news/20190614/Mutant-bacterial-receptor-could-help-identify-therapeutic-compounds-that-inhibit-quorum-sensing.aspx. (accessed July 19, 2019).

  • Harvard

    PLOS ONE. 2019. Mutant bacterial receptor could help identify therapeutic compounds that inhibit quorum sensing. News-Medical, viewed 19 July 2019, https://www.news-medical.net/news/20190614/Mutant-bacterial-receptor-could-help-identify-therapeutic-compounds-that-inhibit-quorum-sensing.aspx.

Comments

The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News-Medical.Net.
Post a new comment
Post
You might also like... ×
New computational model explores daily pain sensitivity rhythms