Study finds first direct evidence of AMR bacteria migrating from the gut to the lungs

A new study released today in Nature Communications from the Department of Biology, University of Oxford has found the first direct evidence of antibiotic-resistant bacteria migrating from a patient's gut microbiome to the lungs. Applying the findings of this study could save lives, as it highlights the importance of preventing pathogenic bacteria from translocating from the gut to other organs where they can cause serious infections.

The study was conducted on a patient that carried the bacterium Pseudomonas aeruginosa as part of their gut microbiome. This species is one of the leading causes of infections in hospitals, and one that is particularly good at resisting antibiotics. Whilst Pseudomonas is generally not considered to be dangerous when it is embedded in a healthy gut microbiome, it can cause serious infections in the lungs of hospitalized patients.

During their stay in hospital the patient was treated with the antibiotic Meropenem for a suspected urinary tract infection (UTI). Meropenem treatment caused non-resistant bacteria in the gut and lung to be killed off, and antibiotic-resistant mutants of Pseudomonas were able to grow and proliferate.

Pseudomonas was then found to translocate from the gut to the patient's lungs during antibiotic treatment, where it evolved even higher levels of antibiotic resistance.

With AMR being an increasing concern in hospitals, preventing the spread of AMR bacteria to other vital organs such as the lung is critical in vulnerable patients. However, the origin of bacteria that cause these serious infections can be difficult to determine. This study shows how the gut microbiome can act as a reservoir of AMR pathogens that can translocate to the lung where they have the potential to cause life-threatening illnesses such as pneumonia.

The findings of this study suggest that eliminating AMR pathogens from the gut microbiome of hospitalized patients could help to prevent serious infections, and it highlights how antibiotic use can have profound impacts on bacteria that are not actually the target of antibiotic treatment.

Researchers tested the patient throughout their time in hospital to track the duration of their infection with Pseudomonas. They used a genetic approach, creating a time-calibrated bacterial family tree that allowed them to analyze the progression and location of infection, plus its evolution. They also found high genetic diversity in the gut, which also suggests that the microbiome may be a reservoir for AMR to arise.

Fortunately, the patient in this case had an immune response to the AMR bacteria in their lungs, preventing the infection from causing pneumonia. However, many people in critical conditions, particularly over winter, have a weakened immune system, meaning the body is less able to fight off disease. AMR policies often focus on reducing infection from external sources, but understanding how AMR can develop and spread within a patient is just as vital.

The researchers now intend to assess how frequently gut to lung bacterial translocation occurs in vulnerable patients by collecting samples from a much larger cohort.

There is a clear need to develop new approaches to the challenges that antimicrobial resistance presents. Our study shows how gut-lung translocation and antibiotic use can combine to drive the spread of AMR within a single patient. Insights such as this are needed in order to develop new interventions to prevent resistant infections. For example, our study highlights a potential benefit of eliminating AMR bacteria like Pseudomonas aeruginosa from the gut microbiome of hospitalized patients, even when these bacteria are not actually causing infection."

Craig MacLean, Professor, Department of Biology

'AMR pathogens can be difficult to eliminate from patients once they have become established, and our work also underscores the importance of avoiding unnecessary antibiotic use, and in developing antibacterial treatments that only target the bacteria that are actually causing an infection.'

Source:
Journal reference:

Wheatley, R.M., et al. (2022) Gut to lung translocation and antibiotic mediated selection shape the dynamics of Pseudomonas aeruginosa in an ICU patient. Nature Communications. doi.org/10.1038/s41467-022-34101-2.

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