Lollipop-based saliva collection system shows promise for more enjoyable, accurate diagnostic testing

A lollipop might be a sweet reward for a kid who's endured a trip to the doctor's office, but now, this candy could make diagnostic testing during a visit less invasive and more enjoyable. Researchers publishing in ACS' Analytical Chemistry have shown, for the first time, that a lollipop-based saliva collection system can capture bacteria from adults and remain shelf-stable for up to a year. Study participants also preferred the candies over conventional collection systems.

Throat swabs are commonly used to collect samples for the diagnosis of a wide variety of illnesses, including strep throat. A less-gag-inducing method is saliva sampling, in which technicians analyze a patient's spit with methods such as quantitative polymerase chain reaction (qPCR). Because this type of sample can be collected directly by a patient, the technique is popular for at-home testing and saw expanded use during the COVID-19 pandemic. Gathering the necessary amount of saliva can be somewhat gross, though, which is why some scientists are looking to make the process more enjoyable by combining it with the equally drool-filled, yet much more pleasant, experience of enjoying a lollipop.

Previously, Sanitta Thongpang, Ashleigh Theberge, Erwin Berthier and colleagues developed their own lollipop collection device dubbed CandyCollect. At first glance, CandyCollect looks like most lollipops, except for its spoon-like stick with a spiral-shaped groove carved into the top. This flattened end is covered with isomalt candy, allowing for saliva to easily flow into the groove as the lollipop is eaten. In a past study, the researchers showed in lab tests that the device could capture the bacteria responsible for strep throat. Now, they wanted to target other, naturally occurring bacteria and see how their system compared to other commercially available, at-home saliva sampling methods with real people.

Researchers sent CandyCollect and two conventional saliva sampling kits to 28 adult volunteers, who used them, answered some survey questions, then shipped the devices back to the lab. The researchers eluted the samples and then quantified Streptococcus mutans and Staphylococcus aureus bacteria using qPCR. Whenever one or both of the conventional methods detected the target bacteria, CandyCollect also detected them 100% of the time. Additionally, the candies were the most popular method of the three among participants, who also agreed it was the "most sanitary" and "least disgusting." The devices still produced accurate results after being stored for a year. Although the studies are still ongoing, the team says that this work shows that the system is adaptable and well liked. The researchers say it could inspire other scientists to develop more intuitive and convenient at-home testing methods.

Source:
Journal reference:

Tu, W-c., et al. (2023) At-Home Saliva Sampling in Healthy Adults Using CandyCollect, a Lollipop-Inspired Device. Analytical Chemistry. doi.org/10.1021/acs.analchem.3c00462.

Comments

The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
Post a new comment
Post

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.

You might also like...
AI model revolutionizes dementia diagnosis with high accuracy across multiple data sources