Researchers explore natural SARS-CoV-2 infection in pet rabbits in France

In a recent study posted to the journal Veterinary Sciences, researchers reported the first evidence of natural severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections among domestic rabbits in France.

Study: First Evidence of Natural SARS-CoV-2 Infection in Domestic Rabbits. Image Credit: Evgeniy Goncharov photo/ShutterstockStudy: First Evidence of Natural SARS-CoV-2 Infection in Domestic Rabbits. Image Credit: Evgeniy Goncharov photo/Shutterstock


Following the emergence of SARS-CoV-2 in late 2019, many reports have indicated that SARS-CoV-2 crosses species-level barriers to cause experimental or natural infection in several domestic and wild animals that come in contact with humans.

A recent study has identified white-tailed deer as a reservoir of SARS-CoV-2, raising the possibility of the emergence of novel zoonotic SARS-CoV-2 reservoirs and viral strains with unknown pathology and subsequently significant public health issues. However, studies that report incidences of SARS-CoV-2 infections in rabbits, both wild and domesticated, are limited.

About the study

In the present study, the researchers collected blood samples of 144 pet rabbits with unknown SARS-CoV-2 status during their veterinary visits to evaluate the presence of antibodies against SARS-CoV-2 from November 2020 to June 2021, covering the peaks of the second and third epidemic waves of SARS-CoV-2 in France.

These blood samples were collected in ethylenediaminetetraacetic acid (EDTA) tubes at the veterinary hospitals. Following centrifugation, the serum samples were stored at +4°C, before being transported to the veterinary diagnostic laboratory, VEBIO, following appropriate safety guidelines to prevent contamination during the shipment and ensuring they reached the laboratory within two days. 

An aliquot of the sample was taken for biomedical analyses at VEBIO, and another sample aliquot was kept at +4 °C before being transported under optimal safety conditions to MIVEGEC lab, Montpellier, for conducting serological analyses. Before testing, these samples were maintained at -20 °C at the MIVEGEC laboratory.

The SARS-CoV-2-specific antibodies in serum samples of rabbits were evaluated using a multiplex microsphere immunoassay (MIA), that used10 µg beads of two recombinant SARS-CoV-2 antigens, trimeric spike (tri-S), and receptor-binding domain (RBD) antigens. The SARS-CoV-2 antigens were coupled with distinct MagPlex microsphere sets using the amine coupling kit and following incubation of the microsphere mixtures, measurements were recorded using a Luminex 200 instrument.


The results indicated that nearly 53% of the rabbits were males, 41% were females, and gender information was unavailable for 6% of rabbits. The mean age of the rabbits was 4.4 years, ranging from one to eight years.

Microsphere assay results showed that of all the 144 samples, one female rabbit was seropositive for S RBD and tri-S antigens and another female rabbit was positive only for S RBD antigen.

Due to the absence of pre-pandemic sera and the hypothesis of less frequent natural SARS-CoV-2 infection among domestic rabbits, the seropositivity in the microsphere assay used a cutoff of three standard deviations higher than all other samples' average signal. 

Among the seropositive rabbits, SARS-CoV-2-associated symptoms were not observed by the veterinarians during sample collection. However, intermittent vestibular syndrome was present among double seropositive rabbits, which was already frequent among rabbits. The seroprevalence was 0.7% using the highest restrictive positivity criterion on the two antigens positive results, and it was 1.4% after a positive result for at least one antigen.


According to the authors, this is the first-ever study demonstrating natural SARS-CoV-2 infection in pet rabbits, with a seroprevalence of 0.7-1.4%, most likely acquired from their SARS-CoV-2-positive owners. Although antibody detection alone does not necessarily indicate active viral replication, the study findings were in line with previous studies that demonstrated the active SARS-CoV-2 replication in rabbits after experimental infection without any signs of clinical manifestations of the disease. 

In previous experiments with rabbits, a relatively higher viral dose was required to develop productive SARS-CoV-2 infection and was associated with a lower viral replication. Another experimental study conducted on three wild-caught cottontail rabbits infected with SARS-CoV-2 failed to establish a productive infection or presence of neutralizing antibodies against SARS-CoV-2.

However, in the present study, a very low SARS-CoV-2 seroprevalence was observed among the 144 domestic rabbits, indicating the lower susceptibility of rabbits to SARS-CoV-2 infection, which may be due to the infrequent contact between the owner and pet rabbits compared to other pet animals, such as dogs and cats.

Overall, the study demonstrated that SARS-CoV-2 infections are rare in rabbits, and they are unlikely to act as a reservoir for SARS-CoV-2 or aid the epidemic spread of SARS-CoV-2 in humans. Since these results were based on the evaluation of pet rabbits, future studies focusing on farm and wild rabbits are required to determine the spread of SARS-CoV-2 in different settings.

Journal reference:
Shanet Susan Alex

Written by

Shanet Susan Alex

Shanet Susan Alex, a medical writer, based in Kerala, India, is a Doctor of Pharmacy graduate from Kerala University of Health Sciences. Her academic background is in clinical pharmacy and research, and she is passionate about medical writing. Shanet has published papers in the International Journal of Medical Science and Current Research (IJMSCR), the International Journal of Pharmacy (IJP), and the International Journal of Medical Science and Applied Research (IJMSAR). Apart from work, she enjoys listening to music and watching movies.


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