Innovative genomic approach identifies rare Long QT syndrome carriers

An innovative analysis of shared segments within the genome -; an indication of distant "relatedness" -; has identified undiagnosed cases of Long QT syndrome, a rare disorder that can lead to abnormal heart rhythms, fainting and sudden cardiac death. 

The findings, reported in the journal Nature Communications, illustrate the feasibility of the new approach developed by researchers at Vanderbilt University Medical Center to detect undiagnosed carriers of rare disease-causing genetic variants. 

Rare genetic diseases are usually studied in referral populations -; people who have been referred to specialty clinics for evaluation -; but this approach often overestimates the true population impact, which would be better assessed in large non-referral populations, such as biobanks."

Jennifer (Piper) Below, PhD, professor of Medicine in the Division of Genetic Medicine and senior corresponding author of the new study 

Because most biobanks recruit participants from the same region, there is often significant undocumented relatedness among the participants, resulting in genomic segments that are shared due to common ancestry -; "identical-by-descent" segments, Below explained. 

"Identical-by-descent segments give us an opportunity to cluster related people to find rare variants that were present in a common ancestor," she said. 

To do this, the researchers developed a genetic inference method called DRIVE (Distant Relatedness for Identification and Variant Evaluation). The studies were led by co-first authors Megan Lancaster, MD, PhD, a clinical fellow in the Division of Cardiovascular Medicine, and Hung-Hsin Chen, PhD, who was a postdoctoral fellow in the Division of Genetic Medicine. Dan Roden, MD, the Sam L. Clark, MD, PhD Chair and Senior Vice President for Personalized Medicine, is co-senior author. 

To test DRIVE, the researchers focused on a rare variant in the gene KCNE1 that causes Type 5 Long QT syndrome (LQT5). The KCNE1 gene encodes a protein that modifies potassium currents. 

An international consortium of 26 centers had identified 89 probands (affected individuals who are the first subjects of a genetic study) with possible LQT5, 140 additional carrier relatives, and 19 cases of another syndrome attributed to variants in KCNE1. 

Of 35 probands with the most common KCNE1 variant (p.Asp76Asn), nine (26%) were evaluated by the Genetic Arrhythmia Clinic at VUMC. None of the probands were known to be related. Three relatives of the probands were also found to carry the variant. 

"This enrichment of a rare variant at VUMC relative to other centers in the consortium suggested that these local probands may be distantly related and that we could use that relatedness to identify additional carriers in BioVU," Below said. BioVU is VUMC's DNA biobank linked to de-identified electronic health records. 

The team first estimated the genome-wide relatedness of the 12 clinically identified p.Asp76Asn carriers and constructed lineage pedigrees. They found eighth to ninth degree relatedness among these pedigrees (for reference, fourth cousins -; great-grandchildren of first cousins -; are ninth degree relatives), supporting the hypothesis of a local common ancestor with the p.Asp76Asn variant. 

Then, the researchers identified shared genomic regions that spanned the KCNE1 gene and applied DRIVE to 69,819 BioVU subjects. They identified 22 BioVU subjects with the shared region, confirmed the p.Asp76Asn variant by DNA sequencing, and assessed electrocardiograms and medical records for features of LQT5. 

Both referral and non-referral carriers of the variant have prolonged QT interval compared to controls. 

"In this study, we used DRIVE to rapidly pinpoint 22 carriers of a previously described pathogenic gene variant," Below said. "DRIVE could also be used to identify unknown causal gene variants, by clustering individuals with shared identical-by-descent segments and assessing the enrichment of disease within clusters. 

"We're excited about the potential of DRIVE to identify undiagnosed cases of genetic disease." 

Co-first author Chen is now a tenure-track assistant research fellow of the Institute of Biomedical Science at Academia Sinica in Taiwan and holds a joint faculty appointment at VUMC. Other authors of the Nature Communications study include Benjamin Shoemaker, MD, Matthew Fleming, MD, PhD, Teresa Strickland, James Baker, Grahame Evans, Hannah Polikowsky, David Samuels, PhD, and Chad Huff, PhD. The research was supported in part by the National Institutes of Health (grants R01GM133169, R01HL159557, P50GM115305, U01HG011181, T32HG008962, T32GM007569, T32GM145734) and the American Heart Association.

Source:
Journal reference:

Lancaster, M. C., et al. (2024). Detection of distant relatedness in biobanks to identify undiagnosed cases of Mendelian disease as applied to Long QT syndrome. Nature Communications. doi.org/10.1038/s41467-024-51977-4.

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