Dual pathways found for centromere specification, ensuring accurate chromosome segregation

Despite the immense amount of genetic material present in each cell, around three billion base pairs in humans, this material needs to be accurately divided in two and allocated in equal quantities. The centromere, located in the middle of each chromosome, is known as the site where cellular equipment attaches to divide chromosomes successfully, but the specific mechanisms behind this remain unknown.

In a major new study reported in The EMBO Journal, researchers at The University of Osaka have identified an additional pathway by which the DNA-packaging histone CENP-A associates with and specifies the location of the centromere. This process is vital for ensuring chromosomes are structured and genes are expressed appropriately.

The study was based around the Holliday Junction Recognition Protein (HJURP), a so-called chaperone protein that ushers centromere-identifying cell components to the correct site on chromosomes. They found that HJURP did not localize at the centromere when the expression of both of two cell components, CENP-C and Mis18C, were eliminated following a double knockout process.

Although it was known that Mis18C recognizes the chaperone HJURP to enable CENP-A's deposition onto centromeres, we found that CENP-C can actually occupy Mis18C's role in this process, providing a parallel pathway that helps ensure successful and timely mitosis or meiosis. We also identified the particular residues of HJURP that enable its binding to CENP-C."

Tatsuo Fukagawa, senior author

The team then built on these findings through analyses in DT40 chicken cells, confirming that these interactions are essential for centromere function during cell division. These analyses showed that when HJURP and CENP-C did not interact, this led to errors in mitosis, slowing cell growth. The combination of no interaction and the removal of Mis18C meant that CENP-A could not be incorporated into chromatin, preventing cellular machinery from knowing the supposed location of the centromere.

"Our work reveals that this sequence-independent epigenetic mechanism of centromere specification has greater diversity than previously thought," explains lead author, Tetsuya Hori. "Given how biologically fundamental the processes of mitosis and meiosis are, our finding that the cell has independent pathways for flagging the location of each chromosome's centromere is valuable."

The key findings of this work, regarding the existence of dual pathways for recruiting HJURP for CENP-A deposition, can provide a solid foundation for future studies on the mechanisms behind centromere functioning and on diseases involving errors of cell division.