UV light induces new types of DNA damage that may cause malignant melanoma

Studies conducted in yeast show that exposure to ultraviolet light (UV) induces new types of DNA damage that may cause the deadliest form of skin cancer, malignant melanoma.

That's according to research conducted by a team featuring undergraduate students from Washington State University's School of Molecular Biosciences. The work, led by WSU researchers John Wyrick and Steven Roberts of the College of Veterinary Medicine, was published today, Nov. 17, in Cell Reports.

While melanoma has been associated with UV light, this study directly links UV exposure to the atypical mutations known to spread the disease. The results also indicate that UV light can induce a more diverse spectrum of mutations than previously suspected.

There's been this debate: how much does UV light cause the mutations that actually cause the cancer? Our research supports that UV light plays a major role in producing mutations specific to the growth and spread of melanoma."

John Wyrick, Researcher, Washington State University

As in humans, UV light damages DNA and produces mutations in baker's yeast, making it an excellent model to study how dangerous the resulting mutations can be.

Researchers irradiated yeast cells from 150 yeast colonies with UV lamps 15 times for 8-second intervals over the course of a month. They used UV-C radiation, the strongest form or ultraviolet light. The team then used whole-genome sequencing to identify an estimated 50,000 mutations that occurred in cells due to the UV irradiation.

The team found about half of all the mutations found in the irradiated cells were rare mutations linked to melanoma.

Until now, UV damage in DNA was thought to be confined to two of the DNA bases: C (cytosine) or T (thymine). However, this new research indicates that UV damage also occurs at low frequency at A (adenine) DNA bases, a surprising finding.

WSU students and researchers also showed that a single intense exposure of ultraviolet-B radiation, which is present in sunlight, could also induce the atypical mutations.

Haley Morris, an undergraduate biochemistry major, began working on the research as a first-year student.

"This has a lot of real-world applications and could help identify causes of severe skin cancers," Morris said. "It's cool that something I enjoy and am a part of has the potential to help a lot of people."