Exposing Cells to the Conditions of Ischemia-Reperfusion While Oxygenation is Monitored by the CLARIOstar with ACU

Life-threatening diseases such as myocardial infarction, stroke, or renal failure can occur due to interrupted oxygen flow. Cells and tissues are temporarily deprived of O2 (ischemia) and develop survival-strategies.

Additionally, upon re-oxygenation (reperfusion), cells are exposed to an additional threat by sudden oxygen increase. Despite being critical for survival, sudden reperfusion of tissue creates an inflammatory response and oxidative stress..

A trial set-up which mimics rapid fluctuations in O2 level is needed for assessment of biological effects of ischemia-reperfusion.

A microplate reader with software-regulated CO2 and O2 flow is employed in the ischemia-reperfusion model shown here. Fluctuations in oxygenation of Cor.4U® cardiomyocytes and HepG2 cells were recorded using an intracellular oxygen-sensitive fluorescent probe .

Read the full article including the methods, results and discussion.

The CLARIOstar® with ACU exposes cells to ischemiareperfusion conditions and monitors their oxygenation

About BMG Labtech

BMG LABTECH has been committed to producing microplate readers for more than twenty years. By focusing on the needs of the scientific community, the company’s innovative microplate readers have earned the company the reputation of being a technology leader in the field.

BMG LABTECH has developed a wide range of dedicated and multi-mode microplate readers for life sciences applications and high-throughput screening.

All BMG LABTECH microplate readers are "Made in Germany" and are conceived, developed, assembled, and tested entirely at our headquarters in Germany.

Since our establishment in Offenburg, Germany in 1989, BMG LABTECH has expanded to offer a worldwide sales and support network with offices in the USA, UK, Australia, Japan and France. Our subsidiaries, regional offices and distributors are committed to bringing you innovative microplate reader technology with the quality and reliability you expect from a German company.

Our staff includes engineers and scientists from the fields of biology, biochemistry, analytical chemistry, and physics.

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Last updated: Jul 30, 2018 at 10:54 AM

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