Measuring Double-Stranded DNA with Fluorescent Dyes: Qubit on BMG Labtech Instruments

Despite being a routine laboratory activity, the quantification of DNA is crucial for subsequent applications like next generation sequencing (NGS). Here the FLUOstar® Omega microplate reader is used instead of the Qubit™ handheld fluorometer for assessment of the broadly utilized Qubit™ nucleic acid stains.

The DNA quantification with Qubit chemistry and Qubit™ fluorometer offers a quick, easy, and intuitive method to estimate dsDNA but is restricted in throughput as only one specimen can be evaluated at a time. The Qubit device determines fluorescence directly in specimen preparation tubes, and the DNA-concentration is subsequently calculated based on two standards measured simultaneously.

The utilization of Qubit® dsDNA HS fluorophore to measure dsDNA on a BMG LABTECH microplate reader is described in this application note. The Qubit kit read on the CLARIOstar®, FLUOstar® Omega, and PHERAstar® obtained a high linearity (R2 > 0.9999).

It also yielded the measurement of DNA dilutions of 50 pg/μl-100 ng/μl. Using Qubit chemistry on BMG LABTECH microplate readers enhances throughput, and at the same time retains the simplicity of the measurement technique.

Read the full article including the methods, results and discussion.

Quantifying double-stranded DNA with fluorescent dyes

BMG LABTECH GmbHAbout BMG Labtech

BMG LABTECH has been committed to producing microplate readers for more than twenty years. By focusing on the needs of the scientific community, the company’s innovative microplate readers have earned the company the reputation of being a technology leader in the field.

BMG LABTECH has developed a wide range of dedicated and multi-mode microplate readers for life sciences applications and high-throughput screening.

All BMG LABTECH microplate readers are "Made in Germany" and are conceived, developed, assembled, and tested entirely at our headquarters in Germany.

Since our establishment in Offenburg, Germany in 1989, BMG LABTECH has expanded to offer a worldwide sales and support network with offices in the USA, UK, Australia, Japan and France. Our subsidiaries, regional offices and distributors are committed to bringing you innovative microplate reader technology with the quality and reliability you expect from a German company.

Our staff includes engineers and scientists from the fields of biology, biochemistry, analytical chemistry, and physics.


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Last updated: Jul 30, 2018 at 10:35 AM

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