Measuring Double-Stranded DNA with Fluorescent Dyes: Qubit on BMG Labtech Instruments

Despite being a routine laboratory activity, the quantification of DNA is crucial for subsequent applications like next generation sequencing (NGS). Here the FLUOstar® Omega microplate reader is used instead of the Qubit™ handheld fluorometer for assessment of the broadly utilized Qubit™ nucleic acid stains.

The DNA quantification with Qubit chemistry and Qubit™ fluorometer offers a quick, easy, and intuitive method to estimate dsDNA but is restricted in throughput as only one specimen can be evaluated at a time. The Qubit device determines fluorescence directly in specimen preparation tubes, and the DNA-concentration is subsequently calculated based on two standards measured simultaneously.

The utilization of Qubit® dsDNA HS fluorophore to measure dsDNA on a BMG LABTECH microplate reader is described in this application note. The Qubit kit read on the CLARIOstar®, FLUOstar® Omega, and PHERAstar® obtained a high linearity (R2 > 0.9999).

It also yielded the measurement of DNA dilutions of 50 pg/μl-100 ng/μl. Using Qubit chemistry on BMG LABTECH microplate readers enhances throughput, and at the same time retains the simplicity of the measurement technique.

Read the full article including the methods, results and discussion.

Quantifying double-stranded DNA with fluorescent dyes

About BMG Labtech

BMG LABTECH has been committed to producing microplate readers for more than twenty years. By focusing on the needs of the scientific community, the company’s innovative microplate readers have earned the company the reputation of being a technology leader in the field.

BMG LABTECH has developed a wide range of dedicated and multi-mode microplate readers for life sciences applications and high-throughput screening.

All BMG LABTECH microplate readers are "Made in Germany" and are conceived, developed, assembled, and tested entirely at our headquarters in Germany.

Since our establishment in Offenburg, Germany in 1989, BMG LABTECH has expanded to offer a worldwide sales and support network with offices in the USA, UK, Australia, Japan and France. Our subsidiaries, regional offices and distributors are committed to bringing you innovative microplate reader technology with the quality and reliability you expect from a German company.

Our staff includes engineers and scientists from the fields of biology, biochemistry, analytical chemistry, and physics.


Sponsored Content Policy: News-Medical.net publishes articles and related content that may be derived from sources where we have existing commercial relationships, provided such content adds value to the core editorial ethos of News-Medical.Net which is to educate and inform site visitors interested in medical research, science, medical devices and treatments.

Last updated: Feb 21, 2020 at 9:39 AM

Citations

Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    BMG LABTECH GmbH. (2020, February 21). Measuring Double-Stranded DNA with Fluorescent Dyes: Qubit on BMG Labtech Instruments. News-Medical. Retrieved on April 25, 2024 from https://www.news-medical.net/whitepaper/20180614/Measuring-Double-Stranded-DNA-with-Fluorescent-Dyes-Qubit-on-BMG-Labtech-Instruments.aspx.

  • MLA

    BMG LABTECH GmbH. "Measuring Double-Stranded DNA with Fluorescent Dyes: Qubit on BMG Labtech Instruments". News-Medical. 25 April 2024. <https://www.news-medical.net/whitepaper/20180614/Measuring-Double-Stranded-DNA-with-Fluorescent-Dyes-Qubit-on-BMG-Labtech-Instruments.aspx>.

  • Chicago

    BMG LABTECH GmbH. "Measuring Double-Stranded DNA with Fluorescent Dyes: Qubit on BMG Labtech Instruments". News-Medical. https://www.news-medical.net/whitepaper/20180614/Measuring-Double-Stranded-DNA-with-Fluorescent-Dyes-Qubit-on-BMG-Labtech-Instruments.aspx. (accessed April 25, 2024).

  • Harvard

    BMG LABTECH GmbH. 2020. Measuring Double-Stranded DNA with Fluorescent Dyes: Qubit on BMG Labtech Instruments. News-Medical, viewed 25 April 2024, https://www.news-medical.net/whitepaper/20180614/Measuring-Double-Stranded-DNA-with-Fluorescent-Dyes-Qubit-on-BMG-Labtech-Instruments.aspx.

Other White Papers by this Supplier

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.