Fluorescence Polarization Dependent Assay for High Throughput, Fast and Precise Quantification of Fc & IgG Containing Derivatives

The manufacture and development of most medicinal antibodies require an accurate, quick and high-throughput analysis of IgG. For development of each probable biopharmaceutical therapeutic, a huge number of specimens need to be analyzed, and monoclonal antibodies have become significantly popular in this domain.

The development of an innovative, quick, and simple fluorescence polarization (FP) based experiment for high-throughput titer assessment of Fc and IgG-containing derivatives is described here. A fluorescently-tagged protein G is utilized in this method. Polarization of emitted light increases once bound protein G is bound to IgG or Fc.

The polarization change is recognized to track the presence of IgG with FP-compatible microplate readers. This method (ValitaTITER) allows a high-throughput, simple and accurate measurement of IgG. BMG LABTECH’s multi-mode plate readers the PHERAstar®, CLARIOstar®, and POLARstar®, display an excellent assay quality. Also, the assay can be carried out utilizing specimen straight cell culture supernatant. It implies that no complicated steps are needed for specimen preparation.

About BMG Labtech

BMG LABTECH has been committed to producing microplate readers for more than twenty years. By focusing on the needs of the scientific community, the company’s innovative microplate readers have earned the company the reputation of being a technology leader in the field.

BMG LABTECH has developed a wide range of dedicated and multi-mode microplate readers for life sciences applications and high-throughput screening.

All BMG LABTECH microplate readers are "Made in Germany" and are conceived, developed, assembled, and tested entirely at our headquarters in Germany.

Since our establishment in Offenburg, Germany in 1989, BMG LABTECH has expanded to offer a worldwide sales and support network with offices in the USA, UK, Australia, Japan and France. Our subsidiaries, regional offices and distributors are committed to bringing you innovative microplate reader technology with the quality and reliability you expect from a German company.

Our staff includes engineers and scientists from the fields of biology, biochemistry, analytical chemistry, and physics.


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Last updated: Mar 28, 2019 at 5:33 AM

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