Observing Extracellular Acidification and Oxygen Consumption in Real-Time to Evaluate Changes in Cellular Metabolism

Abnormality in cellular energy metabolism is a common cause in a variety of conditions including obesity and diabetes as well as cancer. To evaluate mitochondrial function, current technologies measure oxygen consumption rate (OCR) and extracellular acidification (ECA). These methods may overlook the effect of O2 concentration on cellular bioenergetics.

The employment of the CLARIOstar® with atmospheric control unit (ACU) in combination with advanced dyes from Agilent is explained here for estimation of glycolytic flux and O2 consumption in an open-flow respirometry system. Measurement of respiration rate at a steady state is allowed, where O2 given equals O2 utilized.

The Mitochondrial Stress Test (ACU format) by Cayman Chemical coupled with CLARIOstar and ACU gives a "push button" multiplexed method. It has the potential for assessing additional cell health outcomes. Tests employ cell suspensions or adherent cells and can be carried out under user defined [O2] level.

About BMG Labtech

BMG LABTECH has been committed to producing microplate readers for more than twenty years. By focusing on the needs of the scientific community, the company’s innovative microplate readers have earned the company the reputation of being a technology leader in the field.

BMG LABTECH has developed a wide range of dedicated and multi-mode microplate readers for life sciences applications and high-throughput screening.

All BMG LABTECH microplate readers are "Made in Germany" and are conceived, developed, assembled, and tested entirely at our headquarters in Germany.

Since our establishment in Offenburg, Germany in 1989, BMG LABTECH has expanded to offer a worldwide sales and support network with offices in the USA, UK, Australia, Japan and France. Our subsidiaries, regional offices and distributors are committed to bringing you innovative microplate reader technology with the quality and reliability you expect from a German company.

Our staff includes engineers and scientists from the fields of biology, biochemistry, analytical chemistry, and physics.

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Last updated: Jul 31, 2018 at 2:57 AM

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