Observing Extracellular Acidification and Oxygen Consumption in Real-Time to Evaluate Changes in Cellular Metabolism

Abnormality in cellular energy metabolism is a common cause in a variety of conditions including obesity and diabetes as well as cancer. To evaluate mitochondrial function, current technologies measure oxygen consumption rate (OCR) and extracellular acidification (ECA). These methods may overlook the effect of O2 concentration on cellular bioenergetics.

The employment of the CLARIOstar® with atmospheric control unit (ACU) in combination with advanced dyes from Agilent is explained here for estimation of glycolytic flux and O2 consumption in an open-flow respirometry system. Measurement of respiration rate at a steady state is allowed, where O2 given equals O2 utilized.

The Mitochondrial Stress Test (ACU format) by Cayman Chemical coupled with CLARIOstar and ACU gives a "push button" multiplexed method. It has the potential for assessing additional cell health outcomes. Tests employ cell suspensions or adherent cells and can be carried out under user defined [O2] level.

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All BMG LABTECH microplate readers are "Made in Germany" and are conceived, developed, assembled, and tested entirely at our headquarters in Germany.

Since our establishment in Offenburg, Germany in 1989, BMG LABTECH has expanded to offer a worldwide sales and support network with offices in the USA, UK, Australia, Japan and France. Our subsidiaries, regional offices and distributors are committed to bringing you innovative microplate reader technology with the quality and reliability you expect from a German company.

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Last updated: Feb 21, 2020 at 9:39 AM

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