New England Biolabs has expanded its line of PCR reagents to include OneTaq™ and OneTaq Hot Start DNA Polymerases. Optimized blends of Taq and Deep Vent® DNA Polymerases, these new products offer robust amplification across a wide range of templates, including routine, AT-rich and GC-rich amplicons. The 3´–5´ exonuclease activity of Deep Vent increases the fidelity and robustness of Taq, while the optimized buffers further enhance yield and product purity, regardless of a template's GC content.
In addition, OneTaq Hot Start DNA Polymerase utilizes aptamer technology; NEB's unique aptamer blocks polymerase activity at temperatures below 45 degrees C, and the polymerase is activated during normal cycling conditions. Available in convenient product formats, including master mixes, OneTaq shows exceptional performance against other commercially available polymerases, especially when amplifying difficult or GC-rich templates.
As part of its commitment to customer service and product excellence, NEB is inviting researchers to sample OneTaq and OneTaq Hot Start, submit comments with data, and earn a chance to win a free iPad®. NEB will use the feedback to aid in the development of future PCR reagent offerings.
"OneTaq DNA Polymerase represents a powerful solution to the vast majority of endpoint PCR needs," states Dr. Fiona Stewart, Product Marketing Manager for New England Biolabs. "Additionally, OneTaq Hot Start offers the convenience of setting up reactions at room temperature without a separate activation step."
SOURCE New England Biolabs