Lipid fragmentation is class and ion mode specific. For example fragment spectra of phosphocholines yield mainly the characteristic head group fragment (184.07 m/z). MS/MS data acquired in negative ion mode provides information about the fatty acid side chains. Therefore, confident lipid identification requires positive and negative ion mode fragment information. MetaboScape enables merging of data acquired in positive and negative mode and hence enables to easily and confidently identify lipids.
Learn more in the Application Note “Investigating the increased lifespan in C. elegans daf-2 mutants by 4D-Lipidomics” (https://www.bruker.com/fileadmin/user...) how 4D-Lipidomics empowered by PASEF and MetaboScape enabled the assignment of more than 1000 lipids in C. elegans lipid extracts.
Run time: 4:54 mins
4D-Lipidomics-Confident lipid identification based on pos. and neg. ion mode fragment information
Video Credit: Bruker Life Sciences Mass Spectrometry