Using Ion Chromatography and Subsequent UV/VIS Detection to Determine Nitrite and Nitrate in Tobacco

Introduction

The appearance of tobacco-specific nitrosamines is facilitated by the nitrite present in tobacco. Since most of these nitrosamines are carcinogenic, it is essential to determine the presence of nitrite content in tobacco.

This application note describes the application of ion chromatographic separation and subsequent UV/VIS detection after sequential suppression to determine the presence of nitrite and nitrate contents in acetic acid extracts of tobacco.

Sample

Tobacco extract (5% acetic acid)

Sample preparation

Dilution 1:10 in ultrapure water.

Columns

. .
Metrosep A Supp 7 - 250/4.0 6.1006.630
Metrosep A Supp 4/5 - Guard/4.0 6.1006.500

Solutions

Eluent 3.6 mmol/L sodium carbonate
Regenerant 100 mmol/L sulfuric acid
Rinsing STREAM

Analysis

Sequential suppression followed by UV/VIS detection.

Parameters

. .
Flow rate 0.7 mL/minutes
Injection volume 20 μL
Pmax 15 MPa
Recording time 25 minutes
Column temperature 45 °C
Wavelength 220 nm

Instrumentation

940 Professional IC Vario ONE/SeS/PP 2.940.1500
944 Professional UV/VIS Detector Vario 2.944.0010
858 Professional Sample Processor 2.858.0020
MSM Rotor A 6.2832.000
Adapter sleve for Suppressor Vario 6.2842.020

Results

Anions Concentration [mg/kg] RSD [%, n = 3]
1 Acetate - -
2 Nitrite 0.23 8.9
3 Nitrate 337 1.0

Metrohm AG

About Metrohm

Metrohm is a leading manufacturer of high-precision instruments for chemical analysis, who offer a complete line of analytical laboratory and process systems for titration, ion chromatography, spectroscopy, and electrochemistry.

Manufactured in Switzerland since 1943, Metrohm's instruments have a unique reputation for their robust design and outstanding quality. With thousands of installations worldwide, we are the established leader in titration

From the beginning, Metrohm's sales engineers guide you through the instrument purchasing process. Together with our team of application chemists they create unique solutions for your laboratory needs. From installation to routine preventative maintenance, their global team of certified service professionals ensures ongoing performance and reliability of your Metrohm instrument.

Whether it is replacing an existing instrument, developing a new analytical solution or answering your technical questions – Metrohm are here for you. Our local presence, with offices or subsidiaries in over 120 countries, makes Metrohm your neighbor. Metrohm is a familiar face and shares your professional background.


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Last updated: Apr 4, 2018 at 9:40 AM

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