The appearance of tobacco-specific nitrosamines is facilitated by the nitrite present in tobacco. Since most of these nitrosamines are carcinogenic, it is essential to determine the presence of nitrite content in tobacco.
This application note describes the application of ion chromatographic separation and subsequent UV/VIS detection after sequential suppression to determine the presence of nitrite and nitrate contents in acetic acid extracts of tobacco.
Tobacco extract (5% acetic acid)
Dilution 1:10 in ultrapure water.
|Metrosep A Supp 7 - 250/4.0
|Metrosep A Supp 4/5 - Guard/4.0
||3.6 mmol/L sodium carbonate
||100 mmol/L sulfuric acid
Sequential suppression followed by UV/VIS detection.
|940 Professional IC Vario ONE/SeS/PP
|944 Professional UV/VIS Detector Vario
|858 Professional Sample Processor
|MSM Rotor A
|Adapter sleve for Suppressor Vario
||RSD [%, n = 3]
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