Using Pulsed Amperometric Detection to Determine Carbohydrates in Orange Juice

Orange juice includes inositol along with typical sugar components. Inositol acts as the basis for signaling and messenger molecules. This article discusses the application of pulsed amperometric detection (PAD) to determine inositol as well as glucose, sucrose, and fructose contents in orange juice on a Metrosep Carb 2 - 150/4.0 column.

Sample

Orange juice

Sample preparation

Dilution 1:1000.

Columns

Metrosep Carb 2 - 150/4.0 6.1090.420
Metrosep Carb 2 Guard/4.0 6.1090.500

Solutions

Eluent 100 mmol/L sodium hydroxide
10 mmol/L sodium acetate

Parameters

. .
Flow rate 0.5 mL/min
Injection volume 20 μL
Recording time 20 minutes
Column temperature 30 °C

PAD parameters

Cell Wall-Jet cell
Working electrode Gold
Reference electrode Palladium
Spacer 50 μm
Measuring potential 0.05 V
Measuring duration 100 ms
Cycle duration 550 ms
Measuring range 200 μA
Temperature 35 °C
Mode PAD

Analysis

Pulsed amperometric detection

Instrumentation

930 Compact IC Flex Oven/Deg 2.930.2160
IC Amperometric Detector 2.850.9110
858 Professional Sample Processor 2.858.0020
IC equipment Wall-Jet cell: Carb (Au, Pd) 6.5337.010

Results

Compound Conc. [g/L] RSD (%, n = 12)
1 Inositol 1.5 0.5
2 Glucose 20.6 0.3
4 Fructose 23.2 0.3
5 Sucrose 42.5 0.4

About Metrohm

At Metrohm is one of the world’s most trusted manufacturers of high-precision instruments for chemical analysis. Metrohm was founded in 1943 by engineer Bertold Suhner in Herisau, Switzerland. Today, Metrohm is represented in 120 countries by subsidiaries and exclusive distributors. The global Metrohm Group also includes the Dutch companies Metrohm Applikon and Metrohm Autolab, manufacturers of online analyzers and instruments for electrochemical research, respectively. Recently, the Metrohm Group was joined by Metrohm Raman, a leading manufacturer of handheld Raman spectrometers.

Metrohm is the global market leader in analytical instruments for titration. Instruments for ion chromatography, voltammetry, conductivity, and stability measurement make the Metrohm portfolio for ion analysis complete. Instruments for Near-infrared and Raman spectroscopy are another, strongly growing segment of the Metrohm portfolio.

Metrohm is a problem solver, both in the laboratory and within the industrial process. To this end, the company offers their customers complete solutions, including dedicated analytical instrumentation as well as comprehensive application know-how. More than 30% of the company’s employees at the Metrohm international headquarters in Herisau work in R&D.

Metrohm has been owned 100% by the non-profit Metrohm Foundation since 1982. The Metrohm Foundation, which does not exert any influence on the company’s business operations, sponsors gifted students in the natural sciences, supports charitable and philanthropic purposes and, above all, ensures the independence of the company.


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Last updated: Apr 2, 2019 at 9:19 AM

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