Determination of Metformin Hydrochloride Assay According to USP

Metformin is one of the most commonly used drugs in the treatment of diabetes type 2. It is a biguanide. This application note describes the assay of metformin hydrochloride in compliance with USP using acetic anhydride as solvent.

Sample

Pure metformin hydrochloride powder

Sample Preparation

No sample preparation necessary

Configuration

Solutions

Blank Determination

For the determination of the blank, 4 mL of formic acid and 50 mL of acetic anhydride are placed into a titration beaker. The stirred solution is then titrated with c(HClO4) = 0.1 mol/L in glacial acetic acid until the equivalence point is passed. The volume at which the equivalence point is achieved corresponds to the blank value.  

Analysis of Samples

50 to 75 mg of metformin hydrochloride is weighed accurately into a titration beaker to which 4 mL formic acid is added. After complete dissolution, 50 mL of acetic anhydride is added. Following a pause of 30 seconds for mixing, the solution is titrated with c(HClO4) = 0.1 mol/L in glacial acetic acid until the equivalence point is passed.

Parameters

Results

About Metrohm

At Metrohm is one of the world’s most trusted manufacturers of high-precision instruments for chemical analysis. Metrohm was founded in 1943 by engineer Bertold Suhner in Herisau, Switzerland. Today, Metrohm is represented in 120 countries by subsidiaries and exclusive distributors. The global Metrohm Group also includes the Dutch companies Metrohm Applikon and Metrohm Autolab, manufacturers of online analyzers and instruments for electrochemical research, respectively. Recently, the Metrohm Group was joined by Metrohm Raman, a leading manufacturer of handheld Raman spectrometers.

Metrohm is the global market leader in analytical instruments for titration. Instruments for ion chromatography, voltammetry, conductivity, and stability measurement make the Metrohm portfolio for ion analysis complete. Instruments for Near-infrared and Raman spectroscopy are another, strongly growing segment of the Metrohm portfolio.

Metrohm is a problem solver, both in the laboratory and within the industrial process. To this end, the company offers their customers complete solutions, including dedicated analytical instrumentation as well as comprehensive application know-how. More than 30% of the company’s employees at the Metrohm international headquarters in Herisau work in R&D.

Metrohm has been owned 100% by the non-profit Metrohm Foundation since 1982. The Metrohm Foundation, which does not exert any influence on the company’s business operations, sponsors gifted students in the natural sciences, supports charitable and philanthropic purposes and, above all, ensures the independence of the company.


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Last updated: Apr 3, 2019 at 3:26 AM

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