ACROBiosystem's CelThera™ GMP NK Cell Expansion Kit (Phenol Red-free)

The CelThera GMP NK Cell Expansion Kit (Phenol Red-free) is a serum-free culture medium designed to facilitate the cultivation of human NK cells. It is a medium for maintaining and expanding NK cells that is serum- and animal-origin-free.

Product details

Source: ACROBiosystems

Cat. No. Components Size
GMP-CM3102A CelThera GMP Immune Cell Expansion Medium (Phenol Red-free) 1000 mL
GMP-CM31S2 CelThera GMP Immune Cell Supplement C 8 mL

Note: A complimentary bottle of CelThera GMP Phenol Red Solution (0.5 %) (Cat# GMP-PI1100) is supplied with this kit. For cell culture procedures requiring phenol red indicator, add 1.5 mL of this solution to 1 L of CelThera GMP Immune Cell Expansion Medium (Phenol Red-free) and mix thoroughly.

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ACROBiosystem

Image Credit: ACROBiosystems 

Features

  • Devoid of serum, exogenous growth hormones, and animal origin (AOF)
  • Designed to facilitate high-fold NK cell growth and low-density seeding
  • Suitable for large-scale NK cell expansion
  • Contains only recombinant proteins; no antibiotics were introduced during production
  • High-fold NK cell growth can be achieved using less serum or serum replacements
  • Both cancer-positive and healthy blood samples exhibit high-fold expansion

Storage

CelThera GMP Immune Cell Expansion Medium (Phenol Red-free) remains stable for 18 months when stored at 2 to 8 °C and protected from light.

CelThera GMP Immune Cell Supplement C is stable for 24 months when stored at –20 °C or lower and protected from light.

ACRO Quality Management System

  • QMS (ISO, GMP)
  • Quality Advantages
  • Quality Control Process

Performance data

Application data

Four donor-derived human PBMCs were cultured with NK Cell Expansion Medium for two weeks. The results of the cell expansion showed that NK Cell Expansion Kit (ACROBiosystems) can be comparable with the Competitor NK medium. Notably, the total cells exhibit better expansion in NK Cell Expansion Kit (ACROBiosystems).

Four donor-derived human PBMCs were cultured with NK Cell Expansion Medium for two weeks. The results of the cell expansion showed that NK Cell Expansion Kit (ACROBiosystems) can be comparable with the Competitor NK medium. Notably, the total cells exhibit better expansion in NK Cell Expansion Kit (ACROBiosystems). Image Credit: ACROBiosystems

Four donor-derived human PBMCs were cultured with NK Cell Expansion Medium for two weeks. The results of the cell expansion showed that NK Cell Expansion Kit (ACROBiosystems) can be comparable with the Competitor NK medium. Notably, the NK cells exhibits better expansion in NK Cell Expansion Kit (ACROBiosystems).

Four donor-derived human PBMCs were cultured with NK Cell Expansion Medium for two weeks. The results of the cell expansion showed that NK Cell Expansion Kit (ACROBiosystems) can be comparable with the Competitor NK medium. Notably, the NK cells exhibit better expansion in the NK Cell Expansion Kit (ACROBiosystems). Image Credit: ACROBiosystems

Four donor-derived human PBMCs were cultured with NK Cell Expansion Medium for two weeks. The cell viability results showed that NK Cell Expansion Kit (ACROBiosystems) can be comparable with the Competitor NK medium.

Four donor-derived human PBMCs were cultured with NK Cell Expansion Medium for two weeks. The cell viability results showed that the NK Cell Expansion Kit (ACROBiosystems) can be comparable with the Competitor NK medium. Image Credit: ACROBiosystems

Four donor-derived human PBMCs were cultured with NK Cell Expansion Medium for two weeks. The NK proportional results showed that NK Cell Expansion Kit (ACROBiosystems) can be comparable with the Competitor 2 and higher than that of competitor 1.

Four donor-derived human PBMCs were cultured with NK Cell Expansion Medium for two weeks. The NK proportional results showed that the NK Cell Expansion Kit (ACROBiosystems) can be comparable with the Competitor 2 and higher than that of Competitor 1. Image Credit: ACROBiosystems

Four donor-derived human PBMCs were cultured with NK Cell Expansion Medium for two weeks. The results of the TIGIT ratio, a marker of exhaustion, showed that NK Cell Expansion Kit (ACROBiosystems) can be comparable with the Competitor 2 and lower than that of competitor 1.

Four donor-derived human PBMCs were cultured with NK Cell Expansion Medium for two weeks. The results of the TIGIT ratio, a marker of exhaustion, showed that the NK Cell Expansion Kit (ACROBiosystems) can be comparable with Competitor 2 and lower than that of Competitor 1. Image Credit: ACROBiosystems

iPSC-NKs were cultured in NK Cell Expansion Medium Kit (ACROBiosystems) or NK Cell Expansion Medium Kit (Competitor) for two weeks. The result shows that the expansion efficiency of Acro is higher than that of the competitor.

iPSC-NKs were cultured in NK Cell Expansion Medium Kit (ACROBiosystems) or NK Cell Expansion Medium Kit (Competitor) for two weeks. The results show that the expansion efficiency of Acro is higher than that of its competitor. Image Credit: ACROBiosystems

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