The TNF and TNF receptor superfamilies of proteins include TNF-like cytokine 1A (TL1A) and its receptors, death receptor 3 (DR3) and decoy receptor 3 (DcR3). Human TL1A, His-tagged (TLA-H5243), is expressed in HEK293 cells and comprises amino acids Leu72–Leu251, with a His-tagged N-terminus.
Synonym
TL1A, VEGI, TNFSF15
Source
Human TL1A, His Tag (TLA-H5243) is expressed in human 293 cells (HEK293 cells). It includes AA Leu 72-Leu 251 (Accession No. O95150-1).
Predicted N-terminus: His
Molecular characterization
Image Credit: ACROBiosystems
This protein has a polyhistidine tag at the N-terminus.
The protein's predicted MW is 22.4 kDa. Due to glycosylation, the protein migrates at 26 kDa and 28-30 kDa when measured against the Star Ribbon Pre-stained Protein Marker under reducing (R) conditions (SDS-PAGE).
Endotoxin
Less than 0.001 EU per μg by the LAL method / rFC method.
Purity
>90 % as determined by SDS-PAGE.
Formulation
Lyophilized from a 0.22 μm filtered solution in PBS at pH 7.4 with trehalose as a protectant.
Reconstitution
For optimal performance, adhere to the reconstitution instructions outlined in the CoA.
Storage
For long-term storage, keep the product in a lyophilized condition at -20 °C or below.
Avoid repeated freeze-thaw cycles
This product remains stable after storage at:
- -20 °C to -70 °C for 12 months in lyophilized state
- -70 °C for three months under sterile conditions after reconstitution
ACRO Quality Management System
- Quality Control Process
- QMS (ISO, GMP)
- Quality Advantages
Performance data
SDS-PAGE
Human TL1A, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90 % (With Star Ribbon Pre-stained Protein Marker). Image Credit: ACROBiosystems
SEC-MALS
The purity of Human TL1A, His Tag (Cat. No. TLA-H5243) is more than 85 % and the molecular weight of this protein is around 75-95 kDa verified by SEC-MALS. Image Credit: ACROBiosystems
Bioactivity-ELISA
Immobilized Human DcR3, Fc Tag (Cat. No. TNB-H5255) at 5 μg/mL (100 μL/well) can bind Human TL1A, His Tag (Cat. No. TLA-H5243) with a linear range of 0.3-2 ng/mL (QC tested). Image Credit: ACROBiosystems
Immobilized Human TL1A, His Tag (Cat. No. TLA-H5243) at 2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Monoclonal Anti-TL1A Antibody, Human IgG1 and 4 μg/mL (50 μL/well) Biotinylated Human DR3 Protein, Fc, Avitag (Cat. No. DR3-H82F3) with a half maximal inhibitory concentration (lC50) of 1.976 μg/mL (Routinely tested). Image Credit: ACROBiosystems
Immobilized Human TL1A, His Tag (Cat. No. TLA-H5243) at 10 μg/mL (100 μL/well) can bind Human DR3 Protein, Fc Tag (Cat. No. DR3-H5253) with a linear range of 0.005-0.156 μg/mL (Routinely tested). Image Credit: ACROBiosystems
Bioactivity-SPR
Human DR3 Protein, His Tag (Cat. No. TN5-H52H3) immobilized on CM5 Chip can bind Human TL1A, His Tag (Cat. No. TLA-H5243) with an affinity constant of 45.8 nM as determined in a SPR assay (Biacore 8K) (Routinely tested). Image Credit: ACROBiosystems
Anti-TL1A antibody captured on Protein A Chip can bind Human TL1A, His Tag (Cat. No. TLA-H5243) with an affinity constant of 0.765 nM as determined in a SPR assay (Biacore 8K) (Routinely tested). Image Credit: ACROBiosystems
Human DcR3, Fc Tag (Cat. No. TNB-H5255) captured on Protein A Chip can bind Human TL1A, His Tag (Cat. No. TLA-H5243) with an affinity constant of 0.269 nM as determined in a SPR assay (Biacore 8K) (Routinely tested). Image Credit: ACROBiosystems
Human TL1A, His Tag (Cat. No. TLA-H5243) captured on CM5 chip via anti-His antibody can bind Human DR3 Protein, Fc Tag (Cat. No. DR3-H5253) with an affinity constant of 1.15 µM as determined in a SPR assay (Biacore 8K) (Routinely tested). Image Credit: ACROBiosystems
Bioactivity-CELL BASE
Response to human TL1A protein (FOLD). The DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell was stimulated with serial dilutions of human TL1A protein (Cat.No.TLA-H5243). The max induction fold was approximately 40.12. Image Credit: ACROBiosystems