ACRO's PE-Labeled Human BCMA, His Tag (BCA-HP2H2) is manufactured via site-specific conjugation of PE to Human BCMA, His Tag.
Synonym
TNFRSF17, CD269, BCM, BCMA
Source
The PE-Labeled Human BCMA, His Tag (BCA-HP2H2) is generated through a site-specific conjugation of PE to Human BCMA, His Tag under optimal conditions utilizing proprietary technology. Human BCMA, His Tag is expressed in human 293 cells (HEK293). It comprises amino acids Met 1 to Ala 54 (Accession # Q02223-1).
Predicted N-terminus: Met 1
Molecular characterization
Image Credit: ACROBiosystems
This protein possesses a polyhistidine tag located at the C-terminus. It has an estimated molecular weight of 9.6 kDa.
Conjugate
- PE
- Excitation Wavelength: 488 nm / 561 nm
- Emission Wavelength: 575 nm
Application
This product is NOT suitable for use with the streptavidin detection system.
Formulation
Lyophilized from a solution filtered through a 0.22 μm filter in PBS, containing 0.5 % BSA, at pH 7.4, with trehalose serving as a protectant.
Reconstitution
Certificate of Analysis offers detailed instructions. To achieve optimal performance, adhere to the reconstitution protocol outlined in the CoA.
Storage
For extended storage, the product must be kept in a lyophilized state at -20 °C or below.
It should be shielded from light and should not undergo multiple freeze-thaw cycles.
This product remains stable after being stored at:
- -70 °C for 6 months under sterile conditions after reconstitution.
- -20 °C to -70 °C for 12 months in the lyophilized state.
ACRO Quality Management System
- QMS (ISO, GMP)
- Quality Control Process
- Quality Advantages
Performance data
Bioactivity-FACS
1e6 of the Anti-BCMA CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human BCMA Protein, His Tag (Cat. No. BCA-HP2H2) and negative control protein respectively, PE signal was used to evaluate the binding activity (QC tested). Image Credit: ACROBiosystems