The protein has a calculated molecular weight of 35.0 kDa and migrates at approximately 37–40 kDa.
Synonym
Apolipoprotein E, APOE, Apo-E
Source
Human APOE3 Protein, Tag Free (APE-H5216) is expressed from human 293 cells (HEK293). It has AA Lys 19 - His 317 (Accession # P02649-1). Predicted N-terminus: Lys 19
Molecular characterization
Image Credit: ACROBiosystems
This protein does not possess any "tag".
The protein has a molecular weight (MW) of 35.0 kDa. When calibrated against the Star Ribbon Pre-stained Protein Marker under reducing (R) conditions (SDS-PAGE), the protein migrates at 37-40 kDa as a result of glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90 % as determined by SDS-PAGE.
>90 % as determined by SEC-MALS.
Formulation
Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4, with trehalose as protectant.
Reconstitution
Certificate of Analysis offers specific instructions.
Storage
The product should be stored in a lyophilized state at -20 °C or lower for long term storage.
Avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -70 °C for 3 months under sterile conditions after reconstitution.
- -20 °C to -70 °C for 12 months in the lyophilized state.
ACRO Quality Management System
- QMS (ISO, GMP)
- Quality Advantages
- Quality Control Process
Performance data
SDS-PAGE
Human APOE3 Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90 % (With Star Ribbon Pre-stained Protein Marker). Image Credit: ACROBiosystems
SEC-MALS
The purity of Human APOE3 Protein, Tag Free (Cat. No. APE-H5216) is more than 90 %, and the molecular weight of this protein is around 142-168 kDa, verified by SEC-MALS. Image Credit: ACROBiosystems
Bioactivity-FACS
3e5 of HeLa cells were co-incubated with 50 nM Cy3-labeled LNPs-siRNA in the presence or absence of 3 μg/mL Human Apolipoprotein E / APOE3 Protein, Tag Free (MALS verified) (ACRO Biosystems, Cat. No. APE-H5216), washed, and analyzed with FACS. PE signal was used to evaluate the expression of Cy3+ HeLa cells (Routinely tested). Image Credit: ACROBiosystems