Life Science White Papers

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Life Science White Papers are in-depth articles aimed to educate and inform site visitors interested in life science research. Some of these papers are derived from sources where we have existing commercial relationships. Such articles are clearly labelled as sponsored content and are only accepted when the content is deemed to add value to the core editorial ethos of News Medical.

Ramp Rate Data Alone Not Enough for Evaluation of PCR Thermal Cyclers

In order to compare various thermal cycler speeds, it is not enough to take into account only the heating ramp rate and cooling ramp rate available in the technical specifications, as these data do not give a picture of the actual run times.

From Eppendorf 21 Mar 2016

Dual Block Thermocycler Mastercycler® nexus GX2 Allows PCR Optimization, Offers High Flexibility

Flexible application options are essential for optimal utilization of laboratory instruments in many laboratories. In those laboratories where more than one user is dependent on a single instrument, flexible operation can result in usage frequency.

From Eppendorf 21 Mar 2016

Novel Use of EPR Spectroscopy to Study In Vivo Protein Structure

α-synuclein is a protein found abundantly throughout the brain. It is present mainly at the neuron ends where it is thought to play a role in ensuring the supply of synaptic vesicles in presynaptic terminals, which are required for the release of neurotransmitters to relay signals between neurons. It is critical for normal brain function.

From Bruker BioSpin - NMR, EPR and Imaging 15 Mar 2016

Using Real-Time Infrared Chemical Imaging in Mass Spectrometry Tissue Analysis

Mass spectrometry (MS) is an analytical technique, which can be employed in studies involving the determination of the composition of complex biochemical samples.

From Daylight Solutions Inc 14 Mar 2016

PHERAstar® FSX Plate Reader Helps Improve Throughput of Screening Facilities

Screening facilities need to take necessary measures continuously for increased throughput, reduced sample expenditure and better control over cost.

From BMG LABTECH GmbH 22 Feb 2016

Using Secondary UV-VIS Filter Set to Evaluate the Functionality of Eppendorf BioPhotometer® and BioSpectrometer®

Whenever a sample is measured, the quality of the reading cannot exceed that of the measurement. Thus, it is vital to have a control on the sources of error that negatively impact the measurement or maintain them within defined limits. Errors can have technical causes or may be caused due to improper handling. Incorrect handling errors for photometric quantification includes sample concentration being too high or too low, wrongly programmed method factor, or insufficient sample mixing.

From Eppendorf 18 Feb 2016

Using Eppendorf BioSpectrometer for the determination of enzyme activity and substrate concentration

This article shows how enzymatic activity can be effectively determined by means of the Eppendorf BioSpectrometer. The preliminary measurements were carried out by utilizing the “single λ-continuous” method for optimized measurement process. Based on these outcomes, the exact measurements of the enzyme activity were carried out. Linear regression was used to measure enzyme activities.

From Eppendorf 18 Feb 2016

Troubleshooting Guide for the Determination of Nucleic Acids Using Eppendorf BioPhotometer® D30 and Eppendorf BioSpectrometer®

This guide on troubleshooting will enable users to get reliable results when quantifying nucleic acids through various devices available in the Eppendorf photometer series (Figure 1). Therefore, the important factors which help to attain accurate measurements and problem-solving recommendations are given below. Figure 1 shows a pictorial example of Eppendorf BioPhotometer® D30.

From Eppendorf 18 Feb 2016

Standard Curve Generation for Colorimetric Assay in the Kinetic or Basic Eppendorf BioSpectrometer®

This article shows how the absorbance maxima of Ponceau-S is determined over a wavelength range of 220nm to 800nm using the Eppendorf BioSpectromenter®. The methods of analysis using this BioSpectrometer® are the detection of a maximum at 498nm while utilizing the function, Eppendorf “SpectraZoom”. Using the example of Ponceau-S, the colorimetric measurements were done by generating a standard curve. This standard programmed curve was then utilized to measure the concentration of unknown sample.

From Eppendorf 18 Feb 2016

The PROFILE NMR Technique to Characterize Monoclonal Antibodies

Monoclonal antibodies (MAbs) are an important class of drugs. They have the ability to recognize and bind to almost any molecular structure, giving them incredible potential in drug development. MAbs are primarily used in cancer, inflammatory diseases and infection, and currently represent the largest and fastest growing sector of biologic pharmaceuticals.

From Bruker BioSpin - NMR, EPR and Imaging 16 Feb 2016

Advances in 2D NMR Spectroscopy of Monoclonal Antibodies

Monoclonal antibodies (mAbs) represent one of the fastest growing classes of pharmaceuticals, and look set to be the focus of the biotherapeutics industry into the years to come.

From Bruker BioSpin - NMR, EPR and Imaging 9 Feb 2016

Using Phenom ProX Desktop Scanning Electron Microscope in Microbiology Research

Biofilms are aggregates that are formed by bacteria, which grow on surfaces. Commonly found in environments, biofilms are highly resistant to antibiotics, and as such the mechanism and regulation of biofilms are being intensively studied by scientists across the globe.

From Thermo Fisher Scientific Phenom-World BV 8 Feb 2016

Detailed Considerations for Outfitting Preclinical In Vivo Imaging Laboratory

When setting up a preclinical in vivo imaging laboratory, a number of factors have to be taken into account. These will include the use of dedicated imaging systems and the predicted and accurate workflow of studies that will have to be performed.

From Bruker BioSpin - NMR, EPR and Imaging 1 Feb 2016

Characterization of Worm-Like Micelles Based on Rheology

Exploring the characteristics of worm-like micelles (WLMs) has been the focus of numerous academic and industry-based studies. The applications of WLMs are spread across a broad range of industries, from oil recovery to personal care.

From Malvern Panalytical 21 Jan 2016

15N TROSY NMR for a Fresh View on Protein Macro-Molecules

NMR provides a range of structural and interaction insight that can complement other powerful techniques such as X-ray crystallography, mass spectrometry and electron microscopy to help researchers observe biology in real time at a true molecular level.

From Bruker BioSpin - NMR, EPR and Imaging 21 Jan 2016

Using DSC to Monitor Polyol-Induced Thermal Stability Increase in Antibodies

The steady growth of the biotechnology industry has increased the prominence of the study of protein aggregation. Monitoring and reducing the aggregation of proteins is an important aspect of protein therapeutics because aggregation is the potential cause of immunogenicity and reduced activity.

From Malvern Panalytical 21 Jan 2016

Advantages of Using DSC for Stability Screening of Engineered Proteins

Maintaining the required solubility, stability, efficacy and potency of an engineered biotherapeutic protein is critical after its expression in a recombinant system. Analysis and optimization of the characteristics of biotheraputic proteins such as monoclonal antibodies are performed during the engineering process.

From Malvern Panalytical 21 Jan 2016

Life Science White Papers

Ramp Rate Data Alone Not Enough for Evaluation of PCR Thermal Cyclers

Dual Block Thermocycler Mastercycler® nexus GX2 Allows PCR Optimization, Offers High Flexibility

Novel Use of EPR Spectroscopy to Study In Vivo Protein Structure

Using Real-Time Infrared Chemical Imaging in Mass Spectrometry Tissue Analysis

PHERAstar® FSX Plate Reader Helps Improve Throughput of Screening Facilities

Using Secondary UV-VIS Filter Set to Evaluate the Functionality of Eppendorf BioPhotometer® and BioSpectrometer®

Using Eppendorf BioSpectrometer for the determination of enzyme activity and substrate concentration

Troubleshooting Guide for the Determination of Nucleic Acids Using Eppendorf BioPhotometer® D30 and Eppendorf BioSpectrometer®

Standard Curve Generation for Colorimetric Assay in the Kinetic or Basic Eppendorf BioSpectrometer®

The PROFILE NMR Technique to Characterize Monoclonal Antibodies

Advances in 2D NMR Spectroscopy of Monoclonal Antibodies

Using Phenom ProX Desktop Scanning Electron Microscope in Microbiology Research

Detailed Considerations for Outfitting Preclinical In Vivo Imaging Laboratory

Characterization of Worm-Like Micelles Based on Rheology

15N TROSY NMR for a Fresh View on Protein Macro-Molecules

Using DSC to Monitor Polyol-Induced Thermal Stability Increase in Antibodies

Advantages of Using DSC for Stability Screening of Engineered Proteins

Using Solid-State Standard Additions for Determining Active Ingredients in Solid Pharmaceutical Formulations

Using NIR Spectroscopy for Determining the Moisture, Fat, and Nitrogen in Human Feces

Simple, Rapid, and Accurate Ion Analysis of Pharmaceutical Products with Metrohm Inline Matrix Elimination

E-Cigarettes and Ethanol: A forensic Perspective on Vaping

Understanding Consumer-Consented Data to Optimize Digital Health Programs

The Future is Wearable: Advancing Human Health Through Portable Spectroscopy

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