Japanese researchers suggest link between stimulus response and consciousness

NewsGuard 100/100 Score

Japanese researchers centered at Nagoya University reveal a role for orexin neurons of the hypothalamus when mice respond to painful stimuli, and suggest a link between stimulus response and consciousness.

An animal's response to potentially harmful stimuli is known as nociception, and is determined, to some extent, by the level of consciousness. The absence of nociception or the inability to feel pain can cause an animal to unknowingly incur severe injury, while hypersensitivity to pain prevents quick defense responses to a threat to survival (the fight-or-flight response). Neurons in the hypothalamus that produce the neurotransmitter orexin (a chemical messenger) are known to control nociception, and to play a role in wakefulness and reward processing. Pathways involving orexin neurons are also important during the transition from sleep to wakefulness. However, the precise functioning of orexin neurons in nociception has remained unclear. A research team centered at Nagoya University has now uncovered a role for orexin neurons in the link between nociception and the inability to feel pain (analgesia) in mice. The study was recently reported in Scientific Reports.

The researchers bred mice with orexin neurons that could be selectively and temporally destroyed by a toxin. This specific cell death was controlled by the presence or absence of a chemical added to the animals' food. Mice lacking orexin neurons were found to be more sensitive to nociception than control mice, as demonstrated by pain-related behaviors such as withdrawing their paws from a hot plate or licking paws injected with a chemical irritant. Conversely, the artificial activation of orexin neurons significantly decreased such pain-related behaviors, suggesting its analgesic effect.

Selective removal of orexin neurons also reduced neurons expressing another chemical messenger, dynorphin, within part of the hypothalamus. "This indicates that the analgesic effect of activating orexin neurons could involve the combined action of orexin and dynorphin, or other messengers," first author Ayumu Inutsuka says.

Development of a novel measurement system enabled the researchers to record real-time orexin neuronal activity. "We selectively engineered orexin neurons to express a calcium indicator that could be illuminated by an LED light source, and the resultant fluorescence was detected," corresponding author Akihiro Yamanaka explains. "Strong mechanical stimuli applied to the mouse tail in the form of pinching with forceps brought an increase in the indicator signal. No such response was elicited when weaker stimuli were used, nor when the mice were anesthetized."

Temperature-dependent increases of heat stimuli also induced a similar response, which again could not be detected under anesthesia, suggesting that anesthetics may function by inhibiting orexin neurons.

Comments

The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
Post a new comment
Post

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.

You might also like...
Could vitamin D levels be associated with lower back pain?