Mounting media and sera for blocking are first-rate testing agents that can support the cell-imaging experiments.
Fluoroshield is an aqueous mounting medium that can prevent fast photobleaching of fluorescent labels that are most commonly used. The medium has a unique formulation that aids the prevention of fast photobleaching. In addition, fluorescence of the sample is retained during prolonged storage at 4 °C in the dark.
CyGEL™ is an innovative thermoreversible hydrogel that makes it possible for non-adherent cells and organisms to remain immobile. It does the same for their subsequent recovery after microscopy.
CyGEL™ is liquid when cold and a gel at ~21 °C. It is optically clear with low autofluorescence. For imaging experiments longer than 2 hours, Abcam recommends CyGEL Sustain™, which is specially formulated to allow the addition of RPMI and similar culture media, enabling cells to survive for hours.
Sera for Blocking
Most immunostaining protocols include a blocking step to reduce non-specific binding of the antibody. There are several high-quality sera that can be used, from a number of different species, including goat, guinea pig, donkey, mouse and rabbit. The serum species has to be matched to the species that the secondary antibody was raised in, in order for blocking to occur.
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