Mounting Media and Blocking Sera for Cell Imaging

Mounting media and sera for blocking are first-rate testing agents that can support the cell-imaging experiments.

Mounting Media

Fluoroshield is an aqueous mounting medium that can prevent fast photobleaching of fluorescent labels that are most commonly used. The medium has a unique formulation that aids the prevention of fast photobleaching. In addition, fluorescence of the sample is retained during prolonged storage at 4 °C in the dark.

CyGEL™ is an innovative thermoreversible hydrogel that makes it possible for non-adherent cells and organisms to remain immobile. It does the same for their subsequent recovery after microscopy.

CyGEL™ is liquid when cold and a gel at ~21 °C. It is optically clear with low autofluorescence. For imaging experiments longer than 2 hours, Abcam recommends CyGEL Sustain™, which is specially formulated to allow the addition of RPMI and similar culture media, enabling cells to survive for hours.

Sera for Blocking

Most immunostaining protocols include a blocking step to reduce non-specific binding of the antibody. There are several high-quality sera that can be used, from a number of different species, including goat, guinea pig, donkey, mouse and rabbit. The serum species has to be matched to the species that the secondary antibody was raised in, in order for blocking to occur.

Selected References

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Chung CY  et al. Progressive Proximal-to-Distal Reduction in Expression of the Tight Junction Complex in Colonic Epithelium of Virally-Suppressed HIV+ Individuals. PLoS Pathog10:e1004198 (2014).

Hernandez-Delgadillo R  et al. Bismuth oxide aqueous colloidal nanoparticles inhibit Candida albicans growth and biofilm formation. Int J Nanomedicine 8:1645-52 (2013).

Guo Y  et al. PEG-Like Nanoprobes: Multimodal, Pharmacokinetically and Optically Tunable Nanomaterials. PLoS One 9:e95406 (2014).

Najm FJ  et al. Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells. Nat Biotechnol 31:426-33 (2013).

Witasp A  et al. Elevated circulating levels and tissue expression of pentraxin 3 in uremia: a reflection of endothelial dysfunction. PLoS One 8:e63493 (2013).

Dzinic SH  et al. Identification of an intrinsic determinant critical for maspin subcellular localization and function. PLoS One 8:e74502 (2013).

About Abcam

Abcam is a global life sciences company providing highly validated antibodies and other binders and assays to the research and clinical communities to help advance the understanding of biology and causes of disease.

Abcam’s mission is to serve life scientists to help them achieve their mission faster by listening to their needs, continuously innovating and improving and by giving them the tools, data and experience they want. Abcam’s ambition is to become the most influential life science company for researchers worldwide.

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Last updated: Jan 30, 2020 at 5:39 AM


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