Comparing Anti-PEG Binding of Rabbit and Mouse Monoclonal Antibodies

Anti-PEG RabMAb®​ primary antibodies have been demonstrated to show enhanced binding and selectivity when compared to other Anti-PEG antibodies (e.g. Clone AGP3, Vendor A), in both sandwich and direct ELISA assays, for the quantification of PEG and PEG-modified proteins.

These results have been shown to be similar regardless of if it was PEG or PEG-modified targets being detected.

In-house Comparison Data

Comparison of anti-PEG-47 RabMAb® primary antibody (ab51257) and Vendor A mouse MAb (Clone AGP3) in Direct ELISA assay. Goat anti-rabbit IgG-AP used for Anti-PEG-47 detection; goat anti-mouse IgM-AP used for Vendor A MAb detection. Fig. 1a. Direct ELISA using 1 ug/mL of PEG-GCSF. Fig. 1b. Direct ELISA using 1 ug/mL of PEG-IFN.

Figure 1a.

Comparison of anti-PEG-47 RabMAb® primary antibody (ab51257) and Vendor A mouse MAb (Clone AGP3) in Direct ELISA assay. Goat anti-rabbit IgG-AP used for Anti-PEG-47 detection; goat anti-mouse IgM-AP used for Vendor A MAb detection. Fig. 1a. Direct ELISA using 1 ug/mL of PEG-GCSF. Fig. 1b. Direct ELISA using 1 ug/mL of PEG-IFN.

Figure 1b. Comparison of anti-PEG-47 RabMAb® primary antibody (ab51257) and Vendor A mouse MAb (Clone AGP3) in Direct ELISA assay. Goat anti-rabbit IgG-AP used for Anti-PEG-47 detection; goat anti-mouse IgM-AP used for Vendor A MAb detection. Fig. 1a. Direct ELISA using 1 ug/mL of PEG-GCSF. Fig. 1b. Direct ELISA using 1 ug/mL of PEG-IFN.

Comparison of sandwich ELISA using RabMAb®/RabMAb® antibodies (PEG 47/PEG 47, ab51257) and MAb/RabMAb® antibodies (Vendor A/PEG 47). PEG 47/PEG 47: Plated coated with 5 ug/mL of #47; 5 ug/mL of #47 used for detection. Vendor A/ PEG 47: Plates coated with 100 ug/mL of Vendor A Mouse MAb; 5 ug/mL of #47 used for detection. Vendor A = clone AGP3.

Figure 2. Comparison of sandwich ELISA using RabMAb®/RabMAb® antibodies (PEG 47/PEG 47, ab51257) and MAb/RabMAb® antibodies (Vendor A/PEG 47). PEG 47/PEG 47: Plated coated with 5 ug/mL of #47; 5 ug/mL of #47 used for detection. Vendor A/ PEG 47: Plates coated with 100 ug/mL of Vendor A Mouse MAb; 5 ug/mL of #47 used for detection. Vendor A = clone AGP3.

Customer Testimonial

The relatively high (low nM) affinity of the (PEG RabMAb®​ primary antibody, ab53449)  meant that we could extensively wash the wells of the assay (with PBS no Tween) and not remove the specifically bound Ab – this essentially meant we had a ‘sensitive’ assay with ‘low background levels’ and a ‘good signal-to-noise window.

Provided by an Abreview​ 

About Abcam

Abcam is a global life sciences company providing highly validated antibodies and other binders and assays to the research and clinical communities to help advance the understanding of biology and causes of disease.

Abcam’s mission is to serve life scientists to help them achieve their mission faster by listening to their needs, continuously innovating and improving and by giving them the tools, data and experience they want. Abcam’s ambition is to become the most influential life science company for researchers worldwide.


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Last updated: Jun 13, 2019 at 7:51 AM

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