Phosphatase and Protease Inhibitor Cocktails

With the following convenient and accessible phosphatase and protease inhibitor cocktails, you can ensure the protection of your protein samples from degradation while also preserving their phosphorylation state. When undertaking your experiment, there are several inhibitor combinations you can use.

Phosphatase Inhibitor Cocktails

Of all known proteins, roughly 30% can be reversibly phosphorylated. Several cellular processes can be controlled via this method. The predominant phosphorylation points of protein kinases are serine, threonine and tyrosine residues. Phosphatases can reverse this phosphorylation. Consequently, in your samples, it is crucial for you to preserve the phosphorylation state of these proteins during the study of these processes.

Inhibiting several phosphatases - among which are included specific serine/threonine and tyrosine phosphatases, and other non-specific acid and alkaline phosphatases – four phosphatase inhibitor cocktails are listed in the table below.

Kit name Inhibitors included Target protease/phosphatase
Phosphatase Inhibitor Cocktail I β-Glycerophosphate, Sodium fluoride, Sodium orthovanadate, Sodium pyrophosphate decahydrate Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase
Phosphatase Inhibitor Cocktail II Imidazole, Sodium fluoride, Sodium molybdate, Sodium orthovanadate, Sodium tartrate dihydrate Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase
Phosphatase Inhibitor Cocktail III (-)-p-Bromotetramisole oxalate, Cantharidin, Calyculin A Alkaline Phosphatase, Serine/threonine Phosphatase (PP1 and PP2A)
Phosphatase Inhibitor Cocktail IV β-Glycerophosphate, Imidazole, Sodium fluoride, Sodium molybdate, Sodium orthovanadate, Sodium tartrate dihydrate Acid Phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase

Protease Inhibitor Cocktails

Able to degrade proteins as well as affect the sensitivity and reliability of protein expression research, endogenous proteases are contained within cell lysates. Consequently, protease inhibitors are often added to lysate samples as this protects certain proteins of interest. Including serine, cysteine and aspartic proteases, the protease inhibitor cocktails listed in the table below inhibit numerous proteases.

Kit name Key information Inhibitors included Target protease/phosphatase
Protease Inhibitor Cocktail Optimized to generate the maximal protection to mammalian tissue and cell extracts. Aprotinin, Leupeptin, Pepstatin A, PMSF Acidic protease, Cysteine protease, Mammalian acetylcholinesterase, Serine protease
Protease Inhibitor Cocktail (EDTA-free) Ideal for mammalian cell/tissue lysates and bacterial cell extracts using metal chelation chromatography AEBSF hydrochloride, Aprotinin (bovine lung), Bestatin, E-64, Leupeptin hemisulfate, Pepstatin A Aminopeptidase B and leucine aminopeptidase, Aspartic protease, Cysteine protease, Serine protease
Protease Inhibitor Cocktail II General use cocktail AEBSF hydrochloride, Aprotinin (bovine lung), E-64, EDTA disodium salt, Leupeptin hemisulfate Cysteine protease, Metalloprotease, Serine protease
Protease Inhibitor Cocktail III Ideal for bacterial cell extracts using metal chelation chromatography AEBSF hydrochloride, Bestatin, E-64, EDTA disodium salt, Pepstatin A, Aminopeptidase B and leucine aminopeptidase, Aspartic protease, Cysteine protease, Metalloprotease, Serine protease
Protease Inhibitor Cocktail IV Ideal for fungal and yeast cell extracts AEBSF hydrochloride, E-64, Pepstatin A, o-Phenanthroline Aspartic protease, Cysteine protease, Metalloprotease, Serine protease

 

Protease and Phosphatase Inhibitor Cocktails

To provide ultimate protection for your protein samples, combined protease and phosphatase inhibitor cocktails are ideal, and they are available either with or without a metalloproteinase inhibitor known as EDTA.

Kit name Key information Inhibitors included Target protease/phosphatase
Protease and Phosphatase Inhibitor Cocktail AEBSF-free and mass spectroscopy compatible - water soluble Aprotinin (bovine lung), Bestatin, E-64, Leupeptin hemisulfate, β-Glycerophosphate, Sodium fluoride, Sodium orthovanadate, Sodium pyrophosphate decahydrate, EDTA disodium salt Aminopeptidase B and leucine aminopeptidase, Cysteine protease, Metalloprotease, Serine protease, Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase
Protease and Phosphatase Inhibitor Cocktail (EDTA-free) AEBSF-free and mass spectroscopy compatible - water soluble Aprotinin (bovine lung), Bestatin, E-64, Leupeptin hemisulfate, β-Glycerophosphate, Sodium fluoride, Sodium orthovanadate, Sodium pyrophosphate decahydrate Aminopeptidase B and leucine aminopeptidase, Cysteine protease, Serine protease, Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase

 

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Last updated: May 24, 2019 at 7:38 AM

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