Sponsored Content by AbcamFeb 18 2019
With the following convenient and accessible phosphatase and protease inhibitor cocktails, you can ensure the protection of your protein samples from degradation while also preserving their phosphorylation state. When undertaking your experiment, there are several inhibitor combinations you can use.
Phosphatase Inhibitor Cocktails
Of all known proteins, roughly 30% can be reversibly phosphorylated. Several cellular processes can be controlled via this method. The predominant phosphorylation points of protein kinases are serine, threonine and tyrosine residues. Phosphatases can reverse this phosphorylation. Consequently, in your samples, it is crucial for you to preserve the phosphorylation state of these proteins during the study of these processes.
Inhibiting several phosphatases - among which are included specific serine/threonine and tyrosine phosphatases, and other non-specific acid and alkaline phosphatases – four phosphatase inhibitor cocktails are listed in the table below.
| Kit name |
Inhibitors included |
Target protease/phosphatase |
| Phosphatase Inhibitor Cocktail I |
β-Glycerophosphate, Sodium fluoride, Sodium orthovanadate, Sodium pyrophosphate decahydrate |
Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase |
| Phosphatase Inhibitor Cocktail II |
Imidazole, Sodium fluoride, Sodium molybdate, Sodium orthovanadate, Sodium tartrate dihydrate |
Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase |
| Phosphatase Inhibitor Cocktail III |
(-)-p-Bromotetramisole oxalate, Cantharidin, Calyculin A |
Alkaline Phosphatase, Serine/threonine Phosphatase (PP1 and PP2A) |
| Phosphatase Inhibitor Cocktail IV |
β-Glycerophosphate, Imidazole, Sodium fluoride, Sodium molybdate, Sodium orthovanadate, Sodium tartrate dihydrate |
Acid Phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase |
Protease Inhibitor Cocktails
Able to degrade proteins as well as affect the sensitivity and reliability of protein expression research, endogenous proteases are contained within cell lysates. Consequently, protease inhibitors are often added to lysate samples as this protects certain proteins of interest. Including serine, cysteine and aspartic proteases, the protease inhibitor cocktails listed in the table below inhibit numerous proteases.
| Kit name |
Key information |
Inhibitors included |
Target protease/phosphatase |
| Protease Inhibitor Cocktail |
Optimized to generate the maximal protection to mammalian tissue and cell extracts. |
Aprotinin, Leupeptin, Pepstatin A, PMSF |
Acidic protease, Cysteine protease, Mammalian acetylcholinesterase, Serine protease |
| Protease Inhibitor Cocktail (EDTA-free) |
Ideal for mammalian cell/tissue lysates and bacterial cell extracts using metal chelation chromatography |
AEBSF hydrochloride, Aprotinin (bovine lung), Bestatin, E-64, Leupeptin hemisulfate, Pepstatin A |
Aminopeptidase B and leucine aminopeptidase, Aspartic protease, Cysteine protease, Serine protease |
| Protease Inhibitor Cocktail II |
General use cocktail |
AEBSF hydrochloride, Aprotinin (bovine lung), E-64, EDTA disodium salt, Leupeptin hemisulfate |
Cysteine protease, Metalloprotease, Serine protease |
| Protease Inhibitor Cocktail III |
Ideal for bacterial cell extracts using metal chelation chromatography |
AEBSF hydrochloride, Bestatin, E-64, EDTA disodium salt, Pepstatin A, |
Aminopeptidase B and leucine aminopeptidase, Aspartic protease, Cysteine protease, Metalloprotease, Serine protease |
| Protease Inhibitor Cocktail IV |
Ideal for fungal and yeast cell extracts |
AEBSF hydrochloride, E-64, Pepstatin A, o-Phenanthroline |
Aspartic protease, Cysteine protease, Metalloprotease, Serine protease |
Protease and Phosphatase Inhibitor Cocktails
To provide ultimate protection for your protein samples, combined protease and phosphatase inhibitor cocktails are ideal, and they are available either with or without a metalloproteinase inhibitor known as EDTA.
| Kit name |
Key information |
Inhibitors included |
Target protease/phosphatase |
| Protease and Phosphatase Inhibitor Cocktail |
AEBSF-free and mass spectroscopy compatible - water soluble |
Aprotinin (bovine lung), Bestatin, E-64, Leupeptin hemisulfate, β-Glycerophosphate, Sodium fluoride, Sodium orthovanadate, Sodium pyrophosphate decahydrate, EDTA disodium salt |
Aminopeptidase B and leucine aminopeptidase, Cysteine protease, Metalloprotease, Serine protease, Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase |
| Protease and Phosphatase Inhibitor Cocktail (EDTA-free) |
AEBSF-free and mass spectroscopy compatible - water soluble |
Aprotinin (bovine lung), Bestatin, E-64, Leupeptin hemisulfate, β-Glycerophosphate, Sodium fluoride, Sodium orthovanadate, Sodium pyrophosphate decahydrate |
Aminopeptidase B and leucine aminopeptidase, Cysteine protease, Serine protease, Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase |
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