IL-6: A Critical Inflammatory, Pleiotropic Cytokine

IL-6 is a crucial pleiotropic inflammatory cytokine. Increased IL-6 is seen in patients who are suffering from microbial infections and other similar inflammatory conditions. The serum level of IL-6 is usually associated with the severity of the inflammation.

IL-6’s pro-inflammatory function is controlled by a trans-signaling process that involves the gp130 receptor and soluble IL-6R (sIL-6R). TNF-alpha and IL-1beta trigger the production of sIL6R. Both these pro-inflammatory cytokines promote the proteolytic cleavage of IL-6R by stimulating ADAM17 and ADAM10.

sIL-6R is different from most other soluble receptors (for example, IL-18BP and IL-1RA) in that it does not inhibit IL-6. Rather, it forms a complex with gp130 and IL-6 to trigger IL-6 signaling.

Human IL-6R (cat#: 10398-H08H) enhanced the IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 is typically 20–80 ng/mL.

Human IL-6R (cat#: 10398-H08H) enhanced the IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 is typically 20–80 ng/mL. Image Credit: Sino Biological Inc.

Besides this, IL-6 can transduce its signal via membrane-bound IL-6R. For this kind of signal transduction, gp130 also needs to be the co-receptor.

Bioactive recombinant IL-6 from multiple species

Table 1. Source: Sino Biological Inc.

Species Cat# Activity Validated
Human 10395-HNAE Bind to Human IL-6R Cell proliferation
Mouse 50136-MNAE Cell proliferation
Rat 80076-RNAE Cell proliferation
Rhesus 90197-CNAE Cell proliferation
Sus scrofa (Pig) 62006-WNAE Bind to Human IL-6R (10398-H08H)

 

Cell Proliferation Assay Using TF-1 Human Erythroleukemic Cells. (cat#: 10395-HNAE).

Cell Proliferation Assay Using TF-1 Human Erythroleukemic Cells. (Cat#: 10395-HNAE). Image Credit: Sino Biological Inc.

Recombinant membrane-bound IL-6 receptor: IL-6R

IL-6 shows restricted cross-species bioactivity. Human IL-6 can attach to both human and mouse IL-6R; however, the mouse IL-6 can attach only to mouse IL-6R.

ELISA detection pairs: IL-6 and IL-6R

Sino Biological has designed high sensitivity ELISA antibody pairs for the detection of IL-6R and IL-6.

Table 2. Source: Sino Biological Inc.

Target Carture Antibody Detection Antibody
IL-6 10395-MM14 10395-MM72
IL-6R 50280-R033 polyclonal antibody

 

ELISA detection pairs: IL-6 and IL-6R

Image Credit: Sino Biological Inc.

Neutralization antibodies: IL-6 and IL-6R

Neutralize IL-6 induces proliferation in the T1165 mouse plasmacytoma cells. The IC50 is generally 80–320 ng/mL.

IL-6 neutralizing antibody (Cat#: 10395-R508).

IL-6 neutralizing antibody (Cat#: 10395-R508). Image Credit: Sino Biological Inc.

Human IL-6R neutralizing monoclonal antibody neutralizes IL-6-dependent inhibition of cell proliferation. The IC50 is generally 0.15–0.6 μg/mL.

IL-6R neutralizing antibody (Cat#: 10398-MM08).

IL-6R neutralizing antibody (Cat#: 10398-MM08). Image Credit: Sino Biological Inc.

More IL-6 family cytokines

Interleukin 6 (IL6, also called IFNB2, IFN-beta-2, BSF2, BSF-2, IL-6, HSF, HGF and CDF) is found on the choromosome 7p15.3. Interleukin 6 is conserved in Rhesus monkeys, chimpanzees, chickens, rats, mice and dogs.

The gene creates a 23718 Da protein made up of a total of 212 amino acids. As a multifunctional α-helical cytokine, IL6 mediates the growth and differentiation of cells in numerous tissues, which is specifically known for its role in acute phase reactions and the immune response. IL6 plays a crucial role in guiding the change from innate to acquired immunity.

All IL-6 reagents

Table 3. Source: Sino Biological Inc.

. . .
IL-6 Protein (6) Recombinant IL-6 proteins are expressed by E. coli with fusion tags as Native.
IL-6 Antibody (26) IL-6 antibodies are validated with different applications, which are ELISA, Neutralization, WB, IP, ELISA(Cap), ELISA(Det), IHC-P.
IL-6 ELISA Kit (2) IL-6 ELISA Kits are quality controlled by 8 internation QC standards which guarantee every ELISA Kit with high quality.
IL-6 cDNA Clone (66) IL-6 cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each IL-6 of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
IL-6 qPCR Primer (3) Verified forward and reverse primers for analyzing the quantitative expression of gene. The primer mix has been verified to generate satisfactory qPCR data on Roche LightCycler480.
IL-6 IP Kit (1) The Anti-IL-6 magnetic Beads, conjugated with Anti-IL-6 antibody, are used for immuneprecipitation (IP) of IL-6 proteins which expressed in vitro expression systems.

 

IL-6 background

As a multifunctional α-helical cytokine, Interleukin-6 (IL-6) controls the growth and differentiation of cells in numerous tissues. It is specifically known for its role in acute phase reactions and the immune response.

The IL-6 protein is produced by different types of cells, such as macrophages and T cells, as a phosphorylated and unevenly glycosylated molecule. It applies actions through its heterodimeric receptor made up of IL-6R that does not contain the tyrosine/kinase domain and attaches IL-6 with low affinity.

The glycoprotein 130 (gp130) is universally expressed and binds the IL-6. IL-6R complex has an excellent affinity and hence transduces signals.

IL-6 plays a key role in bone metabolism, hematopoiesis and cancer development. It has also been defined as a crucial factor in guiding the change from innate to acquired immunity.

IL-6 references

  1. Heinrich PC, et al. (2003). Principles of interleukin-6-type cytokine signalling and its regulation. Biochem J. 374: 1-20.
  2. Rose-John S, et al. (2007) The IL-6/sIL-6R complex as a novel target for therapeutic approaches. Expert Opin Ther Targets. 11(5): 613-24.
  3. Dinh W, et al. (2009) Elevated plasma levels of TNF-alpha and interleukin-6 in patients with diastolic dysfunction and glucose metabolism disorders. Cardiovasc Diabetol. 8:58.

About Sino Biological Inc.

Sino Biological is an international reagent supplier and service provider. The company specializes in recombinant protein production and antibody development. All of Sino Biological's products are independently developed and produced, including recombinant proteins, antibodies and cDNA clones. Sino Biological is the researchers' one-stop technical services shop for the advanced technology platforms they need to make advancements. In addition, Sino Biological offers pharmaceutical companies and biotechnology firms pre-clinical production technology services for hundreds of monoclonal antibody drug candidates.

Sino Biological's core business

Sino Biological is committed to providing high-quality recombinant protein and antibody reagents and to being a one-stop technical services shop for life science researchers around the world. All of our products are independently developed and produced. In addition, we offer pharmaceutical companies and biotechnology firms pre-clinical production technology services for hundreds of monoclonal antibody drug candidates. Our product quality control indicators meet rigorous requirements for clinical use samples. It takes only a few weeks for us to produce 1 to 30 grams of purified monoclonal antibody from gene sequencing.


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Last updated: May 11, 2021 at 4:12 AM

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