Interleukin-18 (IL-18) is a pro-inflammatory cytokine with several functions. It is a member of the IL-1 superfamily and is involved in both innate and acquired immunity. The IL-18 pathway has been linked to autoimmune illnesses, metabolic problems, and malignancies in recent investigations.
In collaboration with IL-12, IL-18 regulates host immunity by modulating IFN-production. IL-18 stimulates natural killer (NK) cells, natural killer T (NKT) cells, and established Th1 cells to generate IL-3, IL-9, and IL-13 in the presence of IL-2.
IL-18 promotes downstream signal transduction in the same way as IL-1β does. IL18 interacts with the IL-18 receptor α. The downstream cellular signal is initiated once IL-18Rβ/ IL-18RAcP is recruited to the complex. IL-18 signaling is inhibited by IL-18BP, a secreted antagonist receptor. In conjunction with SIGIRR, IL-18Rβ may bind IL-37 and transmit an anti-inflammatory signal.
Comparison between IL-18 and IL-1β
Table 1. Source: Sino Biological Inc
To help in the study of IL-18 biology, Sino Biological has created a panel of recombinant IL-18 and IL-18 receptor protein reagents.
Sino Biological has developed high-quality recombinant IL-18 and receptor proteins from human, mouse, cynomolgus, rhesus, rat, and canine sources.
Ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells in the presence of TNF-alpha Image Credit: Sino Biological Inc.
Batch-to-batch Consistency Image Credit: Sino Biological Inc.
>95% as determined by SEC-HPLC Image Credit: Sino Biological Inc.
Sino Biological has created IL-18 proteins with excellent bioactivity from a variety of species. The function of these proteins has been demonstrated in IFN-γ induction tests and binding assays against IL-18 receptors.
Mouse IL-18 protein (mature form) | 50073-MNCE
Bind with IL-18Rα (Cat#: 50094-M38H) Image Credit: Sino Biological Inc.
Ability to Induce IFN-γ Secretion by KG-1 Human Myelomonocyte Cell Image Credit: Sino Biological Inc.
Recombinant IL-18 receptors
The IL-18 receptor complex is a heterodimer composed of a (IL-18Rα) chain that is responsible for IL-18 extracellular binding and a nonbinding, signal-transducing β (IL-18R β/IL-18RAcP) chain.
Table 2. Recombinant IL-18Rα Source: Sino Biological Inc.
Table 3. Recombinant IL-18Rβ Source: Sino Biological Inc.
Recombinant IL-18 antagonist
Sino Biological has created a panel of antibodies that can be utilized in ELISA, WB, IF, IP, FCM, and ICC to target IL-18 cytokines and receptors.
Table 4. Recombinant Human IL-18BP Source: Sino Biological Inc.
Table 5. Recombinant Mouse IL-18BP Source Sino Biological Inc.
|Recombinant Mouse IL-18BP
Antibodies for IL-18 cytokines and receptors
Sino Biological has established a panel of antibodies that can be utilized in ELISA, WB, IF, IP, FCM, and ICC to target IL-18 cytokines and receptors.
Anti-IL-18Rβ/IL-18RAcP Antibody (Cat#: 10176-T24)
Immunochemical Staining of Human IL-18Rβ in Human Colon Carcinoma Cells Image Credit: Sino Biological Inc.
Anti-IL-18 antibody (Cat#: 310203-T44)
Immunochemical Staining of Human IL-18 in Human Lymph Node Cells Image Credit: Sino Biological Inc.
More related antibodies
IL-18 detection ELISA antibody pair
Sino Biological has designed high-sensitivity ELISA pair sets for IL-18 detection.
- Mouse IL-18 Matched ELISA Antibody Pair Set
- Human IL-18 Matched ELISA Antibody Pair Set
Image Credit: Sino Biological Inc.