VEGFs and VEGF receptors: Key mediators in body systems

Vascular endothelial growth factors (VEGFs) are a family of secreted polypeptides that show angiogenic and mitogenic activities. The significance of VEGFs and receptors in tumorigenesis, angiogenesis, and neuroprotection makes them interesting targets for drug development.

The VEGF family members VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, VEGF-F, and PlGF attach in distinct patterns to three related receptor protein tyrosine kinases —VEGFR1/FLT1, VEGFR2/KDR, and VEGFR3/FLT4 — and two non-tyrosine kinase transmembrane proteins, neuropilin-1 (NRP1) and neuropilin-2 (NRP2).

The ligand-receptor attachment triggers the formation of homo- and heteromeric receptor complexes and enables downstream signaling.

Along with VEGF signaling, neuropilins also serve as co-receptors to various other signaling pathways, like those activated by Fibroblast Growth Factor (FGF), Insulin-like Growth Factor (IGF), Transforming Growth Factor beta (TGFβ), Hepatocyte Growth Factor (HGF), and Platelet-Derived Growth Factor (PDGF).

VEGF family ligands and receptors

More than 65 recombinant VEGF and receptor proteins that cover all VEGF family members and various isoforms of VEGF are provided by Sino Biological.

A great majority of these recombinant proteins are expressed in eukaryotic cells (human cells or insect cells), which affirms the right modification and greater activity of proteins.

High activity

Cell proliferation assay using human umbilical vein endothelial cells (HUVEC).

Cell proliferation assay using human umbilical vein endothelial cells (HUVEC). Image Credit: Sino Biological Inc.

Inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC)

Inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). Image Credit: Sino Biological Inc.

Recommended recombinant VEGF and receptor proteins

Source: Sino Biological Inc.

Cat# Molecule Species Activity Tag
10136-H02H VEGFR1/FLT-1 Human Active hFc
10136-H08H VEGFR1/FLT-1 Human Active His
10136-H08H1 VEGFR1/FLT-1 Human Active His
10806-H02H VEGFR3/FLT4 (ECD) Human Active hFc
50584-M02H VEGFR3/FLT-4 Mouse Active hFc
10012-H02H VEGFR2/KDR Human Active hFc
10012-H02H1 VEGFR2/KDR (Domain 1&2&3) Human Active hFc
10012-H02H1-B VEGFR2/KDR (Domain 1&2&3) Human   hFc
10012-H02H2 VEGFR2/KDR (Domain 2&3) Human Active hFc
10012-H08H2 VEGFR2/KDR (Domain 2&3) Human   His
10012-H20B1 VEGFR2/KDR Human Active GST &
His
50998-M08H-B VEGFR2/KDR Mouse Active His
80113-R02H VEGFR2/KDR Rat Active hFc
80113-R08H VEGFR2/KDR Rat   His
90907-C08H VEGFR2/KDR Cynomolgus   His
10011-H08H NRP1 Human Active His
10695-H02H NRP2 Human Active hFc
11066-HNAB-B VEGF165 Human,
Cynomolgus
  Native
11066-HNAH VEGF165 Human,
Cynomolgus
Active Native
63004-ZNAB VEGF165 Danio rerio
(zebrafish)
Active Native
70004-DNAH VEGF164 Canine Active Native
80006-RNAB VEGF164 Rat Active Native
10544-H01H VEGFB Human Active hFc
80103-R01H VEGFC (aa 108-223) Mouse, Rat Active hFc
80103-R08H VEGFC (aa 108-223) Mouse, Rat Active His
50157-M01H VEGFD Mouse Active hFc
50157-M08H VEGFD Mouse Active His
80104-R01H VEGFD Rat Active hFc
10274-H05H PIGF Human Active mFc

 

More recombinant VEGF and receptor proteins

VEGF/VEGFR antibodies

Sino Biological provides more than 100 antibodies against VEGF and receptor proteins with affirmed activities in numerous applications. The applications include immunohistochemistry, immunofluorescence, western blotting, flow cytometry, neutralization, and ELISA. The company also offers ELISA kits and ELISA pair sets.

 

Sino Biological, Inc.

Video credit: Sino Biological