Rabbit Polyclonal Antibody (pAb) production

Sino Biological provides complete polyclonal antibody production service packages, including peptide or protein antigen synthesis, rabbit immunization, antibody purification, and quality control tests.

These polyclonal antibody services can be tailored to meet a range of different goals. For instance, antisera or purified antibodies can be provided as deliverables. Moreover, multiple purification techniques, such as protein A and antigen affinity purification, can be used to enrich antibody activities. 

Sino Biological promises an ELISA titer exceeding 1:256,000 and has extensive expertise in producing polyclonal antibodies. A variety of service packages are offered to meet the demands of different clients. With the fast development packages, delivery of well-validated rabbit polyclonal antibodies is guaranteed in just 45 days.

Its polyclonal antibody development services are based on years of process optimization and a strong reputation for reliable antibody production. When it comes to antibody development and production, Sino Biological is the partner of choice for the top ten pharmaceutical companies in the world.

Polyclonal antibody production services at a glance

Standard rabbit pAb development package

  • Time: 75 days

Cost-effective using conventional vaccination techniques and a guaranteed ELISA titer of greater than 1:260,000.

Service details

  • Antigen validation (1–2 days)
    • SDS-PAGE and UV analysis
  • Standard immunization (9–12 weeks)
    • Pre-immune bleed
    • 2 rabbits immunization
    • Serum titer test
    • Final bleed
  • Purification (1–2 weeks)
    • Protein A purification
    • Antigen affinity purification (Optional)
  • QC analysis (3 days)
    • Analysis by SDS-PAGE and UV
    • ELISA validation
  • Deliverables
    • Antisera or purified antibodies
    • CoA

Fast rabbit pAb development package

  • Time: 45 days

Service details

  • Antigen validation (1–2 days)
    • SDS-PAGE and UV analysis
  • Fast immunization (6 weeks)
    • Pre-immune bleed
    • 2 rabbits immunization
    • Serum titer test
    • Final bleed
  • Purification (3 days)
    • Protein A purification
    • Antigen affinity purification (Optional)
  • QC analysis (3 days)
    • Analysis by SDS-PAGE and UV
    • ELISA validation
  • Deliverables
    • Antisera or purified antibodies
    • CoA

Featured polyclonal antibody development services

Anti-idiotype antibody development service

To facilitate antibody drug PD/PK and immunogenicity research, three adaptable anti-ID antibody packages are supplied.

Phospho-specific antibody development service

Custom phospho-specific antibodies can be produced as rabbit polyclonal or mouse hybridoma-based monoclonal antibodies upon the user's request.

Featured case studies of custom rabbit polyclonal antibody production

Sino Biological develops polyclonal antibodies for rabbit immunization, antigen production, and antibody purification. The company's rabbit pAb can be used to detect target antigens in IF and IHC assays; Sino Biological validates antibody specificity with knockout HeLa whole cell lysate.

Breast carcinoma.

Breast carcinoma. Image Credit: Sino Biological Inc.

HeLa cells.

HeLa cells. Image Credit: Sino Biological Inc.

Knockout validation.

Knockout validation. Image Credit: Sino Biological Inc.

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