High-quality cytokines for T-cell culture

Tumor-infiltrating lymphocytes and other T cell-mediated treatments, such as CAR-T, have the potential to improve the effectiveness and personalization of cancer care. Cytokines play a crucial part in T cell survival, development, and differentiation.

T cell therapies typically entail the removal of autologous T cells from patients, cell manipulation/engineering, culture expansion, and re-infusion into the patient. A single administration of adoptive T cell therapy can necessitate billions of expanded T cells.

To produce the highest output of T cells while maintaining the correct phenotypic and function, high-quality cytokines must be used.

A powerful cytokine called interleukin-2 (IL-2) is frequently utilized in T cell culture to encourage the proliferation and differentiation of T cells into effector and memory cells.

Other c-cytokines, including IL4, IL-7IL-15, and IL-21, play a vital role in memory T cell development, proliferation, and survival. Varying combinations of IL-2, IL-7, IL-15, and IL-21 have been shown to efficiently expand T cells, resulting in populations expressing early-differentiation markers, such as CD27, CD28, CD62L, and CD127. 

To enhance a particular T cell phenotype, culture conditions can be adjusted. For instance, Th1 needs IL-12, Th2 needs IL-4, Tregs need TGF-β1, and Th17 needs IL-6.

High-quality cytokines for T-cell culture

Image Credit: Sino Biological Inc.

To promote the use of cytokines in T cell-mediated therapies, Sino Biological has created an extensive collection of high-quality cytokines:

  • HPLC Verified
  • High Activity
  • Various Species
  • Low Endotoxin
  • High Purity

Featured Cytokines

Human IL-2 Protein Cat#: 11848-HNAH1-E

High-quality cytokines for T-cell culture

> 95 % as determined by SEC-HPLC. Image Credit: Sino Biological Inc.

High-quality cytokines for T-cell culture

Cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Image Credit: Sino Biological Inc.

Human IL-4 Protein Cat#: 11846-HNAE

High-quality cytokines for T-cell culture

Cell proliferation assay using TF1 human erythroleukemic cells. Image Credit: Sino Biological Inc.

Human IL-7 Protein Cat#: 11821-HNAE

High-quality cytokines for T-cell culture

> 95 % as determined by SEC-HPLC. Image Credit: Sino Biological Inc.

High-quality cytokines for T-cell culture

Cell proliferation assay using anti-CD3 antibody activated human peripheral blood mononuclear cell (PBMC). Image Credit: Sino Biological Inc.

Human IL-21 Protein Cat#: 10584-HNAE

High-quality cytokines for T-cell culture

Ability to induce Interferon-gamma secretion by human natural killer lymphoma NK-92 cells. Image Credit: Sino Biological Inc.

Human IL-6 Protein Cat#: 10395-HNAE

High-quality cytokines for T-cell culture

Cell proliferation assay using TF-1 human erythroleukemic cells. Image Credit: Sino Biological Inc.

High-quality cytokines for T-cell culture

Immobilized Human IL-6 can bind Human IL-6R (Cat: 10398-H02H). Image Credit: Sino Biological Inc.

Human IL-15 Protein Cat#: 10360-HNCE

High-quality cytokines for T-cell culture

Cell proliferation assay using MO7e human megakaryocytic leukemic cells. Image Credit: Sino Biological Inc.

More Cytokines related to T Cell Culture

Source: Sino Biological Inc.

Cat# Species Cytokine Expression Host Tag Purity Activity
11848-H08H Human IL2 HEK293 Cells C-His > 95 %  
11848-HNAH1-E Human   HEK293 Cells Native > 95 % (HPLC verified) Active
51061-MNAE Mouse   E. coli Native > 92 % Active
70014-DNAE Canine   E. coli Native > 95 % Active
80075-RNAE Rat   E. coli Native > 95 % Active
11846-HNAE Human IL4 E. coli Native > 92 % Active
11846-HNAE-B Human   E. coli Native > 90 %  
51084-M08B Mouse   Baculovirus-Insect Cells C-His > 95 % Active
51084-MNAE Mouse   E. coli Native > 90 % Active
70021-DNAE Canine   E. coli Native > 96 % Active
10395-HNAE Human IL6 E. coli Native ≥ 95 % Active
50136-MNAE Mouse   E. coli Native > 90 % Active
62006-WNAE Sus scrofa (Pig)   E. coli Native > 95 % Active
80076-RNAE Rat   E. coli Native > 85 % Active
90197-CNAE Rhesus   E. coli Native > 85 % Active
11821-HNAE Human IL7 E. coli Native > 95 % (HPLC verified) Active
50217-MNAE Mouse   E. coli Native > 90 % Active
80329-RNAE Rat   E. coli Native > 90 % Active
CT011-H08H Human IL12 HEK293 Cells C-His > 95 % Active
CT011-H08H-B Human   HEK293 Cells C-His > 95 %  
CT021-M02H Mouse   HEK293 Cells C-hFc > 85 % Active
CT022-M08H Mouse   HEK293 Cells C-His > 90 % Active
CT023-M0802H Mouse   HEK293 Cells C-His & C-hFc > 85 % Active
CT024-M0208H Mouse   HEK293 Cells C-His & C-hFc > 85 % Active
CT045-C08H Rhesus   HEK293 Cells C-His > 95 %  
CT050-H08H Human   HEK293 Cells C-His > 95 % Active
CT050-HNAH Human   HEK293 Cells Native > 95 % Active
10360-H07E Human IL15 E. coli N-His > 95 % Active
10360-HNCE Human   E. coli N-cleavage > 95 % Active
10584-HNAE Human IL21 E. coli Native > 95 % (HPLC verified) Active
50137-MNAE Mouse   E. coli Native > 97 % Active
70016-DNAE Canine   E. coli Native > 97 % Active
80200-R08B Rat   Baculovirus-Insect Cells C-His > 90 % Active
80200-RNAE Rat   E. coli Native > 95 %  

 

Other Related Proteins

  • CD127
  • CD27
  • BCMA
  • CD62L
  • TGF-β1
  • CD28
  • CD19

Other Equipment by this Supplier

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