ZORBAX HILIC Plus LC Column Agilent

Agilent ZORBAX HILIC Plus columns are designed for hydrophilic interaction chromatography (HILIC) applications. HILIC is typically used for the retention and resolution of small polar compounds. HILIC Plus columns are non-bonded silica columns based on the high performance silica used in the ZORBAX Eclipse Plus columns. This silica provides excellent peak shape, critical for many polar, basic analytes. These columns ship prepared for use in HILIC mode – containing acetonitrile:water – in order to reduce the extensive equilibration typically required for HILIC separations. The HILIC Plus columns are available in a 3.5 µm particle size for high resolution and in 2.1 and 4.6 mm id for compatibility with mass spectrometers or with standard UV detectors.


  • HILIC column for good retention of small, polar analytes
  • Based on Eclipse Plus silica for excellent peak shape
  • High sensitivity for LC/MS applications
  • Recommended for EPA Method 1694